Extraction of furfural inhibitor from biomass hydrolysate of rice husk
Keywords:Furfural, Residual biomass, Liquid-liquid extraction
The production of second generation ethanol (E2G) has proven to be an alternative to non-renewable fuels, through transforming lignocellulosic waste into renewable fuel. In turn, rice husk has great potential due to its availability and composition. The conversion of lignocellulosic biomass to biofuel comprises a fundamental pre-treatment step, however, at this stage, the formation of degradation products (inhibitory compounds) occurs, among them, furfural, which cause negative effects on the viability of fermentative cells, making the production of E2G unfeasible. Given the above, the objective of this work was to remove the furfural inhibitor present in the lignocellulosic broth after the pre-treatment process, using oleic acid, through liquid-liquid extraction. The quantification of total reducing sugars in the hydrolyzate did not show significant variation between the pre and post extraction stages. Regarding the furfural inhibitor, in tests performed with a solution made in the laboratory, removal of up to 62.30% was obtained when the initial concentration was 5.00 g.L-1. With respect to the tests with the hydrolyzate from the rice husk pre-treatment, the maximum removal observed was 10.40%, but the initial concentration of furfural was 1.64 g.L-1. The results obtained indicate the possibility of using oleic acid as an extracting agent of the furfural inhibitor from lignocellulosic hydrolysates.
Almeida, J.R.M.; Modig, T.; Petersson, A. Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. Journal of Chemical Technology and Biotechnology, 82, 340–9, 2017.
Barros, M.R.A.; Cabral, J.M.S.; Novais, J.M.; Production of ethanol by immobilized Saccharomyces bayanus in an extractive fermentation system, Biotechnol. Bioeng. 29 1097–1104, 1987.
Bernfeld, P. Amylases, α and β. Methods in Enzymology, v. 1, p. 149-157, 1955.
BRASIL. Ministério de Minas e Energia; Empresa de pesquisa energética. Balanço energético nacional, 2021.
Canilha, L.; Chandel, A. K.; Suzane Dos Santos Milessi, T.; Antunes, F. A. F.; Luiz Da Costa Freitas, W.; Das Graças Almeida Felipe, M.; Da Silva, S. S. Bioconversion of sugarcane biomass into ethanol: An overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. Journal of Biomedicine and Biotechnology, p. 15, 2012.
Carvalheiro, F.; Duarte, L. C.; Gírio, F. M. Hemicellulose biorefineries: a review on biomass pretreatments. Journal of Scientific & Industrial Research. 67, 849–864, 2008.
Gírio, F.; Fonseca, C.; Carvalheiro, F.; Duarte, L.; Marques, S.; Bogel-Lukasik, R. Bioresource Technology Hemicelluloses for fuel ethanol: A review. Bioresource Technology, 101, 4775–4800, 2010.
Jassal, D.S.; Zhang, Z.; Hill, G.A. In situ extraction and purification of ethanol using commercial oleic acid, Can. J. Chem. Eng. 72, 822-827, 1994.
Jayakody, L.N.; Hayashi, N.; Kitagaki, H. Identification of glycolaldehyde as the key inhibitor of bioethanol fermentation by yeast and genome-wide analysis of its toxicity. Biotechnology Letters, 33, 285–92, 2011.
Khabarov, Y.G., Kamakina, N.D., Gusakov, L.V., Veshnyakov, V.A. A new spectrophotometric method for determination of furfural and pentoses. Russ J Appl Chem, 79, 103–106, 2006.
Khamis, Nor & Shamsudin, Saleha & Rahman, Nur & Kasim, Khairul. Effects of autohydrolysis on rice biomass for reducing sugars production. Materials Today: Proceedings. 16. 2078-2087, 2019.
Lemos, D. A. et al. Selection and application of nontoxic solvents in extractive ethanol fermentation. Biochemical Engineering Journal, 127, 128-135, 2017.
Lemos, D. A. Estudo do processo de fermentação alcoólica extrativa por solventes orgânicos. Tese (Doutorado) – Departamento de Engenharia Química, UFSCar, São Carlos/SP, 2017.
Lemos, D.A.; Sonego, J.L.S.; Cruz, A.J.G.; Badino, A.C. In situ extractive ethanol fermentation in a drop column bioreactor: Extractive ethanol fermentation performed in a drop column bioreactor. Journal of Chemical Technology & Biotechnology. 93. 10.1002/jctb.5504, 2018.
Lemos, D.A.; Sonego, J.L.S.; Cruz, A.J.G.; Badino, A.C. Improvement of ethanol production by extractive fed-batch fermentation in a drop column bioreactor. Bioprocess Biosyst Eng. 2020.
Liu, Z.L. Molecular mechanisms of yeast tolerance and in situ detoxification of lignocellulose hydrolysates. Applied Microbiology and Biotechnology, 90, 809–25, 2011.
Malinowski, J.J. Two-phase partitioning bioreactors in fermentation technology. Biotechnology Advances, 19, 525–538, 2001.
Martin, C.; Marcet, M.; Almazán, O.; Jönssen, L.J. Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors. Bioresource Technology 98, 1767-1773, 2007.
Mota, Claudio J. A.; Monteiro, Robson S.. Química e sustentabilidade: novas fronteiras em biocombustíveis. Quím. Nova, São Paulo, v. 36, n. 10, p. 1483-1490, 2013.
Nakanishi, S.C.; Soares, L.B.; Biazi, L.E.; Nascimento, V.M.; Costa, A.C.; Rocha, G.J.M.; Ienczak, J.L. Fermentation strategy for second generation ethanol production from sugarcane bagasse hydrolyzate by Spathaspora passalidarum and scheffersomyces stipitis. Biotechnol Bioeng, v. 114, n. 10, p. 2211-2221, 2017.
Nakasu, P.Y.S.; Ienczak, L.J.; Costa, A.C.; Rabelo, S.C. Acid post-hydrolysis of xylooligosaccharides from hydrothermal pretreatment for pentose ethanol production. Fuel, v. 185, p. 73-84, 2016.
Offeman, R.D.; Franqui-Espiet, D.; Cline, J.L.; Robertson, G.H.; Orts, W.J. Extraction of ethanol with higher carboxylic acid solvents and their toxicity to yeast. Separation and Purification Technology, 72, 2, 180-185, 2010.
Palmqvist, E.; Hahn-Hägerdal, B.. Fermentation of lignocellulosic hydrolysates II: Inhibitors and mechanisms of inhibition, Bioresource Technology, v. 74, p. 25– 33, 2000.
PERES, J., JUNIOR, E., GAZZONI, D.. Biocombustíveis uma oportunidade para o agronegócio brasileiro. Revista de Política Agrícola, 2005.
Portal Ageitec da EMBRAPA.; 2017. Brasília: Empresa Brasileira de Pesquisa Agropecuária. Disponível em: https://www.agencia.cnptia.embrapa.br/gestor/arroz/arvore/CONT000fe7457q102wx5eo07qw4xezy8czjj.html#. Acesso em: 27 jun. 2021.
Qian, X. H. et al. A initio molecular dynamics simulations of beta-d-glucose and beta-dxylose degradation mechanisms in acidic aqueous solution, Carbohydr. Res. v. 340, p. 2319– 2327, 2005.
Rabelo, S. C. Avaliação e otimização de pré-tratamentos e hidrólise enzimática do bagaço de cana-de-açúcar para a produção de etanol de segunda geração. Tese (Doutorado) – Faculdade de Engenharia Química, UNICAMP, Campinas/SP, 2010.
Reyes, J.; Peralta-Zamora, P.; Durán, N. Hidrólise enzimática de casca de arroz utilizando-se celulases. Efeito de tratamentos químicos e fotoquímicos. Química Nova, 21, 2, 140-143, 1998.
Roque, Laerti & Morgado, Grazielle & Nascimento, Viviane & Ienczak, Jaciane & Rabelo, Sarita. Liquid-liquid extraction: A promising alternative for inhibitors removing of pentoses fermentation. Fuel. 242. 775-787. 10.1016/j.fuel.2018.12.130, 2019.
Santos, Martha Suzana Rodrigues dos. Estudo de pré-tratamentos de palha e sabugo de milho visando a produção de etanol 2G. 2014. 74 f. Dissertação (Mestrado em Engenharia Química) – Centro de Tecnologia, Programa de Pós Graduação em Engenharia Química, Universidade Federal de Alagoas, Maceió, 2014.
Santos, S.C.; Sousa, A.S.; Dionísio, S.R.; Tramontina, R.; Ruller, R.; Squina, F.M.; Vaz Rossell, C.E.; Costa, A.C.; Ienczak, J.L. Bioethanol production by recycled scheffersomyces stipitis in sequential batch fermentations with high cell density using xylose and glucose mixture. Bioresource technology, v. 219, p. 319-329, 2016.
Vargas Betancur, Gabriel J; Pereira JR, Nei. Sugar cane bagasse as feedstock for second generation ethanol production: Part I: Diluted acid pretreatment optimization. Electron. J. Biotechnol., Valparaíso , v. 13, n. 3, p. 10-11, mayo 2010.
Zautsen, R. R. M. Fermentação alcoólica e extração líquido-líquido simultânea de etanol e de inibidores provenientes de caldo hidrolítico de biomassa lignocelulósica.194 p, 2011.
- 2022-04-18 (2)
- 2022-04-04 (1)
How to Cite
Copyright (c) 2022 Ciência e Natura
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.