Merlot wine and the metabolic and cardiovascular changes in LDLR -/- mice

Authors

DOI:

https://doi.org/10.5902/2179460X68358

Keywords:

Functional foods, Resveratrol, Cardiovascular diseases, Insulin, Cholesterol, Dyslipidemia

Abstract

Dyslipidemia is a risk factor for the insulin resistance and cardiovascular diseases, such as left ventricular hypertrophy and remodeling of the arterial. Resveratrol, present in grapes, is a possible mediator of cardiovascular protection. The study aimed to evaluate the effect of red wine from the Merlot grape (Vitis vinifera L.) on dyslipidemia and its involvement in male mice, knockout for the LDL receptor gene (LDLR-/-). The mice were divided into 4 experimental groups: Group S received standard rodent food; Group SV received standard feed for rodents and wine; Group HL received high fat diet; Group HLV received hyperlipidic food and wine orally for 60 days. All animals received water and food ad libitum during this period. After this period, they remained fasting and were then anesthetized. Blood was collected for serum analysis of triglycerides, total cholesterol, and its fractions (HDL and VLDL), C-reactive protein, glucose, and insulin to compare the data. Morphometric and histological analyzes of the left ventricle and abdominal aorta were also performed. It was found that Merlot red wine has antidyslipidemic effects on genetic dyslipidemia, associated with food, partially preventing insulin resistance, hyperinsulinemia and increased CRP; as well as cardiovascular effect, partially preventing LVH and arterial remodeling.

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Author Biographies

Jander Alves dos Anjos, Instituto Federal do Sul de Minas

He holds a degree in Animal Science from the José do Rosário Vellano University, a degree in Veterinary Medicine from the José do Rosário Vellano University, Master's degree in Food Science and Technology from the Federal Institute of Education, Science and Technology of the South of Minas, degree in Biological Sciences from the Faculty of Education Paulistana and doctoral student in the postgraduate program degree in Reproduction, health and animal welfare from the José do Rosário Vellano University, Alfenas, MG, Brazil.

Letícia Albano Hipólito da Silva, Universidade José do Rosário Vellano

Has secondary-high school education at Colégio Athens (2012). She is currently at the José do Rosário Vellano University.

Rafael Pereira Marinho, Universidade José do Rosário Vellano

doctor Rafael Pereira Marinho CRM 81153-MG is a general practitioner. He holds a degree in Medicine from the José do Rosário Vellano-UNIFENAS University, (2019). Currently, he works at Santa Casa de Alfenas-MG as a General Physician in the Emergency Room, Internist in the Internal Medicine and Palliative Care Ward and on duty in the Intensive Care Unit (ICU). 

Wilson Roberto Mesquita Oliveira, Universidade José do Rosário Vellano

Graduated in Bachelor's and Degree in Sciences - Biology from the José do Rosário Vellano University and Master's in Morphological Sciences from the José do Rosário Vellano University. He is currently professor of Dentistry  and Veterinary Medicine, at Universidade José from Rosario Vellano-UNIFENAS. He has experience in the field of Morphology, working mainly on the following topics: Cell Biology, Embryology and Histology.

 

Gersika Bitencourt Santos, Universidade José do Rosário Vellano

Professor at the José do Rosário Vellano University. She holds a degree in Pharmacy from the José do Rosário Vellano University. Master's and Doctorate in Pharmaceutical Sciences from the Federal University of Alfenas (UNIFAL-MG). She has experience in oxidative stress and biochemical tests for the evaluation of anti-inflammatory and antioxidant compounds, in addition to her line of research the study of substances with antidiabetic potential in animal models.

Maria das Graças de Souza Carvalho, Universidade Estadual de Campinas

Bachelor's degree in Biological Sciences from the Federal Institute of Education, Science and Technology of the South of Minas Gerais. Master's student in Reproduction, Health and Animal Welfare at José do Rosário Vellano University (CAPES Scholarship) (2019-2021). Ongoing PhD in Molecular and Morphofunctional Biology at the State University of Campinas (CNPq Scholarship holder). 

Brígida Monteiro Vilas Boas, Instituto Federal do Sul de Minas

Graduated in Agronomy, Master's and Doctorate in Food Science and Post-Doctorate from the Federal University of Lavras. She is currently a Professor at the Federal Institute of Education, Science and Technology of Southern Minas Gerais-Campus Machado. She works in the Professional Master's Program in Food Science and Technology. She has experience in the area of Food Science, with an emphasis on Postharvest of Fruits and Vegetables and Processing of Vegetable Products.

 

Aline Manke Nachtigall, Instituto Federal do Sul de Minas

Graduated in Food Chemistry from the Federal University of Pelotas, Master in Agroindustrial Science and Technology from the Federal University of Pelotas and PhD in Food Science and Technology from the Federal University of Viçosa. She is currently a teacher of basic, technical and technological education at the Federal Institute of Education, Science and Technology of the South of Minas Gerais-Campus Machado. 

Isabela Peregrino, Instituto Federal do Sul de Minas

Graduated in Pharmacy and Biochemistry from the Federal University of Juiz de Fora, graduated in Technology in Viticulture and Enology from the Federal Institute of Education, Science and Technology of Rio Grande do Sul, specialization in Sanitary Surveillance and Quality Control from Estácio de Sá University and Master's in Food from the Federal Institute of Education, Science and Technology of the South of Minas Gerais.

Flávia Helena Pereira, Universidade José do Rosário Vellano

Graduated in Nursing from the School of Nursing of Ribeirão Preto, University of São Paulo, Master in Sciences and Doctorate in Health Sciences from the Graduate Program in Nursing at FEnf-UNICAMP. She is currently a professor of the technical nursing course at the Federal Institute of Sul de Minas. She develops research activities in the area of ​​Nursing with an emphasis on the themes: Process of caring for chronic non-communicable diseases (Diabetes Mellitus type 2), Sleep, Sepsis, Septic Shock, Patient Safety.

José Antônio Dias Garcia, Universidade José do Rosário Vellano

PhD in Functional and Molecular Biology from the State University of Campinas; Master in Morphological Sciences by José do Rosário Vellano University; Specialist in Animal and Human Physiology from the Pontifical Catholic University of Minas Gerais; Specialist in Teaching in Professional and Technological Education; Graduated in Veterinary Medicine from the Faculty of Veterinary Medicine of the José do Rosário Vellano University. Professor and researcher at the José do Rosário Vellano University and at the Federal Institute of Education, Science and Technology in the South of Minas Gerais.

Jefferson Luís Sancineto da Silva Nunes, Enolab laboratory of business consultancy and advice

Technical manager of the Enolab laboratory

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Published

2023-08-21

How to Cite

Anjos, J. A. dos, Silva, L. A. H. da, Marinho, R. P., Oliveira, W. R. M., Santos, G. B., Carvalho, M. das G. de S., Vilas Boas, B. M., Nachtigall, A. M., Peregrino, I., Pereira, F. H., Garcia, J. A. D., & Nunes, J. L. S. da S. (2023). Merlot wine and the metabolic and cardiovascular changes in LDLR -/- mice. Ciência E Natura, 45, e13. https://doi.org/10.5902/2179460X68358

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Vol. 45 (2023): Continuous Publication

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Chemistry

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