Plant cytogenetics tests can predict toxic effects on human cells: genotoxic and mutagenic effects of Tityus serrulatus scorpion venom on vegetal and human cells

Authors

DOI:

https://doi.org/10.5902/2179460X66067

Keywords:

Cytogenotoxicity, mutagenic action, tunel, comet assay, micronuclei

Abstract

The effects of Tityus serrulatus venom was accessed on the cell cycle and genetic material of the Lactuca sativa L. and compared with the damages to human leukocytes, in order to evaluate the genotoxic and mutagenic potential of toxins on cell types very different. The occurrence of cell cycle alterations and DNA fragmentation was evaluated in Lactuca sativa. Incubations of venom with human leukocytes were also held with subsequent evaluation of cell proliferation index, micronucleus and fragmented DNA. The T. serrulatus venom showed cytogenotoxic, reducing mitotic index and induced alterations in the cell cycle and micronuclei formation. The occurrence of cell death was evidenced by the detection of condensed nuclei, positive TUNEL signals, and presence of DNA fragmentation on lettuce cells. The scorpion venom induced DNA fragmentation and micronuclei in leukocytes. Further, the role of peptides and proteases from this venom in inducing the observed damage was discussed.

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Author Biographies

Silvana Marcussi, Universidade Federal de Lavras

Licenciada em Biologia pela Organização Educacional Barão de Mauá (2001), mestre em Biotecnologia pela UNAERP (2003) e doutora em ciências pela FMRP-USP (Departamento de Bioquímica e Imunologia). Pós-doutora em Farmacologia pela FCFRP-USP (Departamento de análises Clínicas, Toxicológicas e Bromatológicas). Tenho experiência nas áreas de Bioquímica, Toxicologia, Citogenética e Farmacologia atuando principalmente nas subárea de toxinologia e produtos naturais: caracterização enzimática, farmacológica e toxicológica de proteínas de peçonhas de animais; plantas medicinais; inibidores enzimáticos, etc. Coordenadora Adjunto do programa de pós graduação em Agroquímica de 2016 a 2017. Atual Profa. Associada IV na Universidade Federal de Lavras, UFLA-MG (início em 2009), locada no Departamento de Química.

Marcel José Palmieri, Universidade Federal de Lavras

Biólogo, mestre e doutor em Genética e Melhoramento de plantas pela Universidade Federal de Lavras (UFLA). Atualmente é pesquisador atuando na área de prospecção de efeitos tóxicos de compostos químicos e biológicos.

Larissa Fonseca Andrade-Vieira, Universidade Federal de Lavras

Bióloga, phD em Ecotoxicologia pela Université de Lorraine, França. Professora Adjunta do departamento de Biologia da Universidade Federal de Lavras (UFLA). Docente e Orientadora do Programa de Pós Graduação em Botânica Aplicada da UFLA. Atua na área de eco(geno)toxidade de poluentes ambientais com projetos conduzidos dentro da tematica "Prospecção de Efeitos Biológicos". Durante o seu mestrado trabalhou com ênfase em citotoxicidade e genotoxicidade de substâncias químicas no ciclo celular. No doutorado atuou com ênfase em citogenética molecular de forrageiras, tendo auxiliado na implementação das técnicas de Citogenética Molecular (FISH e GISH) no Laboratório de Citogenética da UFLA. Como treinamento pos-doutoral realizou ensaios em organismos modelos para ecotoxicologia aquatica conduzindo testes com padronização ISO para acessar a ecotoxicidade de herbicidas. Atualmente tem trabalhado com o modelo vegetal Lactuca sativa para fins de avaliações de toxidade de poluentes ambientais, com ênfase no resíduo da indústria de alumínio, SPL, em agrotóxicos e produtos naturais.

Amanda Ribeiro Barroso, Universidade Federal de Lavras

Graduada em Biologia pela Universidade Federal de Lavras (UFLA). Mestre em Farmacologia pela Universidade Federal do Paraná (UFPR). Possui experiência na área de Toxicologia e Farmacologia.

 

Mariana Aparecida Braga, Universidade Federal de Lavras

Possui graduação em Licenciatura Plena em Química pela Universidade Federal de Lavras (2012). Mestre em Agroquímica, área de concentração em Química/ Bioquímica (2015). Doutora em Agroquímica área de concentração Química/Bioquímica (UFLA). Tem experiência na área de Química, com ênfase em Bioquímica, enzimologia, atuando principalmente nos seguintes temas: tratamento pós-colheita de frutos, plantas medicinais, tratamento da obesidade, determinação de genotoxicidade e mutagenicidade em plantas medicinais, fitoterápicos, inibidores de enzimas digestivas, farmacologia de proteínas de peçonhas de serpentes, plantas antiofídicas e ministração de aulas práticas para graduação.

Marcus Vinicius Cardoso Trento, Universidade Federal de Lavras

Doutorando em Agroquímica (Química e Bioquímica) na Universidade Federal de Lavras (UFLA). Formado em Ciências Biológicas (Bacharelado) e Mestre em Agroquimica (Quimica e Bioquímica de Produtos Naturais e Sintéticos), ambos pela UFLA. Tem experiência na área de Bioquímica, com ênfase em Toxinologia. Participou do programa Ciência Sem Fronteiras na University of The Fraser Valley em Abbostsford, BC, Canadá.

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Published

2023-10-11

How to Cite

Marcussi, S., Palmieri, M. J., Andrade-Vieira, L. F., Barroso, A. R., Braga, M. A., & Trento, M. V. C. (2023). Plant cytogenetics tests can predict toxic effects on human cells: genotoxic and mutagenic effects of Tityus serrulatus scorpion venom on vegetal and human cells. Ciência E Natura, 45, e19. https://doi.org/10.5902/2179460X66067

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Vol. 45 (2023): Continuous Publication

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Biology-Genetics

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