Molecular identification of the main allergens present in household dust of asthmatic patients
Keywords:Asthma, Dust mite, Cockroaches
The main aeroallergens present in house dust are the mites,Dermatophagoidespteronyssinus (Derp) andDermatophagoidesfarinae (Derf), and cockroaches,Periplanetaamericana (Pera). Objective: This work aims to genetically identify the allergens Derp, Derf, and Pera in household dust of asthmatic patients. Materials and methods: 29 patients, aged between 3 and 17 years, were classified as asthmatic or non-asthmatic according to the International Study of Asthma and Allergies in Childhood(ISAAC). Subjectscompleted a complementary questionnaire and skin hypersensitivity tests were performed. House dust was collected from these patients, filtered, and then DNA was extracted. Polymerase chain reactions were performed to identify Derp, Derf, and Pera in the samples. Results: There was an association between Pera sensitization and onset of asthma. There was also an association between the presence of Derp in the home of asthmatic patients and the worsening of symptoms, such as wheezing in the chest and allergic rhinitis. An association between the presence of Derf in house dust of asthmatic patients and the symptoms of allergic rhinitis was found. These data suggest that cockroach sensitization is a predominant factor in asthmatic children and the presence of mite allergens contributesto the worsening of asthma symptoms.
BAIZ N, MACCHIAVERNI P, TULIC MK, REKIMA A, ANNESI-MAESANO I, VERHASSELT V, ET AL. Early oral exposure to house dust mite allergen through breast milk: A potential risk factor for allergic sensitization and respiratory allergies in children. J Allergy Clin Immunol. 2017;139(1):369-72 e10.
BERGMANN, K. C.;RAULFFS, M.; SANDER, I. House dust mites known to be an allergen source for 50 years: Der p 1 still detectable in the original sample. Allergo J. 2014; Int, v.23, n.8, p.320-321.
BERNTON HS, BROWN H. INSECT ALLERGY--PRELIMINARY STUDIES OF THE COCKROACH. J Allergy. 1964 Nov-Dec;35:506-13.
BERTELSEN, R. J. et al. Clinical markers of asthma and IgE assessed in parents before conception predict asthma and hayfever in the offspring. Clin Exp Allergy. May, 2017; v.47, n.5, p.627-638.
CHAN SL, ONG TC, GAO YF, TIONG YS, WANG DY, CHEW FT, et al. Nuclear magnetic resonance structure and IgE epitopes of Blo t 5, a major dust mite allergen. J Immunol. Aμg, 2008; 181(4):2586-96.
CHARPIN, D. High altitude and asthma: beyond house dust mites. Eur Respir J. Dec, 2012; v.40, n.6, p.1320-1.
DUTRA MS, RONCADA C, DA SILVA GL, FERLA NJ, PITREZ PM. Mite Fauna Assessment in Houses of Two distinct Socioeconomic Groups From Southern Brazil. J Med Entomol. 2018;55(3):620-5.
DUTRA, M. S. Avaliação da acarofauna em ecossistemas domiciliares do município de Porto Alegre/RS, Brasil. 2016
FAZLOLLAHI MR, NAJMI M, FALLAHNEZHAD M, SABETKISH N, KAZEMNEJAD A, BIDAD K, et al. Paediatric asthma prevalence: The first national population-based survey in Iran. Clin Respir J. 2019;13(1):14-22.
GIBSON, P. G.; MCDONALD, V. M. Management of severe asthma: targeting the airways, comorbidities and risk factors. Intern Med J. Jun, 2017; v.47, n.6, p.623-631.
GUILLEMINAULT L, VIALA-GASTAN C. Blomia tropicalis: A house dust mite in the tropics. Rev Mal Respir. 2017;34(8):791-801.
HENSZEL, L.; KUZNA-GRYGIEL, W. [House dust mites in the etiology of allergic diseases]. Ann Acad Med Stetin. 2006; v.52, n.2, p.123-7.
JUST, J.;BOURGOIN-HECK, M.; AMAT, F. Clinical phenotypes in asthma during childhood. Clin Exp Allergy. Apr 19, 2017.
LAWSON, J. A. et al. Prevalence, risk factors, and clinical outcomes of atopic and nonatopic asthma among rural children. Ann Allergy Asthma Immunol. Mar, 2017; v.118, n.3, p.304-310.
PAGAN JA, HUERTAS AJ, IRAOLA V, PINTO H, MARTINEZ R, RAMIREZ M, ET AL. Mite exposure in a Spanish Mediterranean region. Allergol Immunopathol (Madr). 2012;40(2):92-9.
POMÉS, A. ARRUDA, LK. Investigating cockroach allergens: aiming to improve diagnosis and treatment of cockroach allergic patients. Methods. 2014 Mar 1;66(1):75-85.
RABITO FA, CARLSON JC, HE H, WERTHMANN D, SCHAL C. A single intervention for cockroach control reduces cockroach exposure and asthma morbidity in children. J Allergy Clin Immunol. 2017 Aug;140(2):565-570.
RIZZO MC, Fernández-Caldas E, Solé D, Naspitz CK. IgE antibodies to aeroallergens in allergic children in São Paulo, Brazil. J InvestigAllergol Clin Immunol. Jul-Aμg, 1997; 7(4):242-8.
SOLARZ, K.; BREWCZYNSKI, P. Z. [Biology of house dust mites (Acari: Astigmata: Pyroglyphidae)]. Wiad Parazytol. 1999; v.45, n.4, p.449-64.
SOLE, D. et al. Asthma in children and adolescents in Brazil:
contribution of the International Study of Asthma and Allergies in Childhood (ISAAC). Rev Paul Pediatr, v.32, n.1, p.114-25, Mar, 2014.
THOMAS WR. Geography of house dust mite allergens. Asian Pac J
Allergy Immunol, Dec, 2010; 28(4):211-24.
THOMAS WR. Geography of house dust mite allergens. Asian Pac J
Allergy Immunol. 2010;28(4):211-24.
TO, T. et al. Global asthma prevalence in adults: findings from the cross-sectional world health survey. BMC Public Health, v.12, p.204, Mar 19, 2012.
WALSH, G. M. Biologics targeting IL-5, IL-4 or IL-13 for the treatment of asthma - an update. Expert Rev Clin Immunol. Feb, 2017; v.13, n.2, p.143-149.
How to Cite
Copyright (c) 2020 Ciência e Natura
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.