Structural characteristics and biotechnological applications of frutalin: lectin extracted from Artocarpus incisa

Bianca Régia Silva, Franscisco Léo Nascimento de Aguiar, José Roberto Viana Silva

Abstract


Frutalin is a lectin extracted from the seeds of Artocarpus incisa, which belongs to the Moreacae family. This family consists of approximately 75 genera and 1,550 tropical species that commonly found in pan-tropical regions. Frutalin has attracted the attention of researchers due to its ability to recognize carbohydrates in cell membranes with modifications in the glycosylation pattern. Therefore, frutalin presents a striking potential to be used as biomarker of cancer cells. Despite having a great biotechnological potential, research involving this lectin is still limited. Thus, the aim of this review is to discuss the structural and functional characteristics of frutalin, properties, the mechanisms of action, as well as the biotechnological applications of this lectin.


Keywords


Artocarpus incisa; Frutalin; Toxicity

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References


ABDON APV, SOUZA GC, SOUZA LNC. Gastroprotective potential of frutalin, α-D-galactose binding lectin, against ethanol-induced gastric lesions. Fitoterapia. 2012;83, 3:604-608.

BRANDO-LIMA AC, et al. Involvement of phosphatidylinositol-3 kinase–Akt and nuclear fator kappa-B pathways in the effect of frutalin on human lymphocyte. International Immunopharmacology. 2006;6:465–472.

BRANDO-LIMA AC, SALDANHA-GAMA RF, HENRIQUES MGMO, MONTEIRO-MOREIRA ACO, MOREIRA RA, BARJA-FIDALGO C. Frutalin, a galactose-binding lectin, induces chemotaxis and rearrangement of actin cytoskeleton in human neutrophils: Involvement of tyrosine kinase and phosphoinositide 3-kinase. Toxicology and Applied Pharmacology. 2005;208:145-154.

BROCKHAUSEN I. Pathways of O-glycan biosinthesis in cancer cells. Biochimica et Biophysica Acta – General Subjects. 1999;1473(1):67-95.

CARVALHO JCT. Fitoterápicos Anti-inflamatórios. Ribeirão Preto: Tecmed, 2015;116:125-131.

CAVALCANTE MDES, et al. A panel of glycoproteins as candidate biomarkers for early diagnosis and treatment evaluation of B-cell acute lymphoblastic leukemia. Biomarker Research. 2016:4-1.

CONSTANCIO APN. Utilização da Frutalina, uma lectina a-D-galactose ligante de Artocarpus incisa L., no estudo dos linfomas de Hodgkin forma clássica, em Ceará/CE [dissertação]. Fortaleza: Universidade Federal do Ceará/UFC, 2005.

DAMASCENO MB, et al. Frutalin reduces acute and neuropathic nociceptive behaviours in rodent models of orofacial pain. Chemico-Biological Interactions. 2016;256:9-15.

FERREIRA MVP. Frutalina, lectina α-D-galactose ligante de Artocarpus incisa L. Um estudo com câncer de mama [thesis]. Fortaleza: Universidade Federal do Ceará/UFC; 2001.

GALLEGO DEL SOL F, NAGANO C, CAVADA BS, CALVETE JJ. The first crystal structure of a Mimosoideae lectin reveals a novel quaternary arrangement of a widespread domain, J. Mol. Biol. 2005;353:574-583.

HAN C, JIN J, XU S, LIU H, LI N, CAO X. Integrin CD11b negatively regulates TLR-triggered inflammatory responses by activating Syk and promoting degradation of MyD88 and TRIF via Cbl-b. Nat. Immunol. 2010;11:734–742.

HARABAYASHI J, KUNO A, TATENO H. Lectin-based structural glycomics: a pratical approach to complex glycans. Electrophoresis. 2011; 32:1118-1128.

JIANG JF, HAN Y, XING LJ, XU YY, XU ZH, CHONG K. Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from. Oryza sativa. Toxicon. 2006;47:133–139.

KHATAM MRI, SABBAG MRPH, NIKRAVAN N, KHAZAEIPOUR Z, BOROUMAND MA, SADEGHIAN S, DAVOUDI B. The role of neutrophil-gelatinase-associated lipocalin in early diagnosis of contrast nephropathy. Indian J Nephrol. 2015;25:292–296.

KIM HJ, LEE SJ. Antibody-based enzyme-linked lectin assay (ABELLA) for the sialylated recombinant human erythropoietin present in culture supernatant. Journal of Pharmaceutical and Biomedical Analysis. 2008;48(3):716–721.

LALWANI AK, CAREY TE, GOLDSTEIN IJ, PETERS BP. Lectin binding characteristics of squamous cell carcinomas of the head and neck. Acta Otolaryngol. 1996;116(1):125-131.

MILHOME MVL, FERREIRA FVA, SCHMITT FCL, ROCHA FILHO F D, MOREIRA RA. Frutalin alpha D galactose binding-lectin,antifrutalin rabbitt antibody (FTLrab),galectin3,CK19 and HBME1 as cyto and histological thyroid carcinoma markers. Revista Médica do Paraná. 2004;62:94.

MONTEIRO ACO. Características químicas, físico-químicas e estruturais da frutalina: uma lectina D-galactose ligante de Artocarpus incisa, em Ceará/CE [dissertação]. Fortaleza: Universidade Federal do Ceará/UFC; 1998.

MONTEIRO-MOREIRA AC, PEREIRA HD, VIEIRA NETO AE, MORENO FBMB, LOBO MDP, SOUSA FD, MOREIRA RA. Crystallization and preliminary X-ray diffraction studies of frutalin, an -D-galactose-specific lectin from Artocarpus incisa seeds. Acta Crystallographica. 2015;7:1282-1285.

MOREIRA RA, AINOUZ IL. Isolectins from jackfruit (Artocarpus integrifolia) seeds. Plant. Physiology. 1978:61-118.

NEPOMUCENO DR. Clonagem e expressão de uma lectina de Artocarpus incisa L. (Frutalina) em Escherichia coli, em Ceará/CE [dissertação]. Fortaleza: Universidade Federal do Ceará/UFC; 2008.

OLIVEIRA C, FELIX W, MOREIRA RA, TEXEIRA JA, DOMINGUES L. Expression of frutalin, an α-d-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris protein expression and purification. Protein Expression and Purification. 2008;60:188–193.

OLIVEIRA C, NICOLAU A, TEXEIRA JA, DOMINGUES L. Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells. Journal of Biomedicine and Biotechnology. 2011:16-10.

OLIVEIRA C, TEIXEIRA JA, SCHMITT F, DOMINGUES L. A comparative study of recombinant and native frutalin binding to human prostate tissues. BMC Biotechnology. 2009:9-78.

OLIVEIRA C, TEXEIRA JA, DOMINGUES L. Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin. Frontiers in Plant Science. 2014;8:5-390.

PEREIRA C, et al. Involvement of AKT and NFkappa-B on lymphocyte activation by frutalin, a galactose-binding lectin. Nitric Oxide, Cytokines and inflammation-An International Symposium. 2004;95:1072-1081

PEREIRA HM, et al. Crystal Structure of Frutalin from Artocarpus incisa. Protein Data Bank. 2015; 227:292-293.

PEUMANS WJ, VAN DAMME EJM. Lectins as plant defense proteins. Plant Physiology. 1995;109(2):347-352.

SILVA WJ, et al. Effects of essential oils on Aedes aegypti larvae: alternatives to environmentally safe insecticides. Bioresour. Technol., 2008; 99:3251-325.

SCHOEPPNER HL, RAZ A, HO SB, BRESALIER RS. Expression of an endogenous galactose-binding lectin correlates with neoplastic progression in the colon. Cancer. 1995;75(12):2818-26.

SHARON N, LIS H. History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology. 2004;14(11):53–62.

CADE O SOARES?

YOON KS, LEE KP, KLOCHKOVA TA, KIM GH. Molecular characterization of the lectin, bryohealin, involved in protoplast regeneration of the marine alga Bryopsis plumosa (Chlorophyta). J Phycol. 2008;44:103–112.

VIEIRA NETO AE. Caracterização estrutural da frutalina, uma lectina a-D-galactose ligante de sementes de Artocarpus incisa e análise das suas bases moleculares de ligação a D-glactose, em Ceará/CE [dissertação]. Ceará: Universidade Federal do Ceará/UFC; 2015.

ZHANG DL, LU CH, YU DH, WANG ZY. Characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker Larimichthys crocea. Fish and Shellfish Immunology. 2016;26(3):3791-810




DOI: http://dx.doi.org/10.5902/2179460X30905

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