Modulação do estado de polimerização in vitro dos filamentos intermediários em astrócitos pela fosforilação e proteína S100B

Authors

  • Erica Bortoli Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS.
  • Juliana K. Frizzo Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS.
  • Carlos Alberto Gonçalves Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS.

DOI:

https://doi.org/10.5902/2179460X27235

Keywords:

GFAP, S100B, protein phosphorylation, glial plasticity.

Abstract

Intermediate filaments represent a major cytoskeleton constituent in eukaryotic cells. GFAP ("glial fibrillary acidic protein") is the monomer of these filaments in astrocytes and its polymerization is apparently modulated by phosphorylation and by interaction with S100B, a calcium-binding protein. In this study we investigate in vitro polymerization of GFAP, using an assay based on imidazol/high magnesium-induced sedimentation. In fact, soluble GFAP (nonpolymerized) increased about 25% in presence of S100B or PKA (protein kinase A). These data suggest that both mechanisms could be acting in the cycle of polymerization/depolymerization of GFAP at different times and/or conditions, therefore affecting glial plasticity.

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References

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Published

2003-12-09

How to Cite

Bortoli, E., Frizzo, J. K., & Gonçalves, C. A. (2003). Modulação do estado de polimerização in vitro dos filamentos intermediários em astrócitos pela fosforilação e proteína S100B. Ciência E Natura, 25(25), 41–50. https://doi.org/10.5902/2179460X27235

Issue

CIÊNCIA E NATURA, V. 25, N. 25, 2003

Section

Articles

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