Ação de extratos vegetais e fitoreguladores na organogênese de Begonia rex

Authors

  • Elcí Terezinha Henz Franco Departamento de Biologia, Centro de Ciências Naturais e Exatas - CCNE Universidade Federal de Santa Maria - UFSM, Santa Maria, RS.
  • Cinara Echart Almeida Departamento de Biologia, Centro de Ciências Naturais e Exatas - CCNE Universidade Federal de Santa Maria - UFSM, Santa Maria, RS.

DOI:

https://doi.org/10.5902/2179460X26964

Keywords:

Begonia rex. micropropagation, organogenesis, in vitro culture.

Abstract

Petiole explants of Begonia rex were cultured on basal medium (MURASHIGE & SKOOG, 1962). The medium was suplemented with naphthalene acetic acid (0.01; 0.1 and 0.5 mg/I) and kinetin ( 0.1; 0.2; 0.5 and 1.0 mg/I). In these experiments, were as also used coconut water (15% and 20%) or potato extract ( 15% and 20%). Buds were formed in several treatments, but the best combination was coconut water 0.01 mg/L NAA and 0.1 mg/I KIN. Whole plants (40% of the explants) were obtained when was added coconut water . The optimal combination for plant regeneration (100%) was 0.01 mg/I NAA plus 0.1 mg/I KIN.

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References

CALDAS, L. S., TORRES, A.C. Meios de cultura. IN: TORRES, A. C., CALDAS, L. S. Técnicas e aplicações de cultura de tecidos de plantas. Brasília: ABCTP/ Embrapa - CNPH, 1990. p. 433.

CASTILLO, B. & SMITH, M. A L. - Direct embryogenis from Begonia gracilis explants. Plant Cell Report, n 16, p. 385-388, 1997.

GRATIAPAGLlA, D., MACHADO, M. A Micropropagação. In: TORRES, A. C., CALDAS, L. S. Técnicas e aplicações da cultura de tecidos de plantas. Brasília: ABCTP/Embrapa-CNPH, 1990. p. 99-169.

HILDING, A, WELANDER, T. Effects of some Factors on Propagation of Begonia x hiemalis in vitro. Swedish J. Agric. Res.n. 6, p. 191-199, 1976.

HU, C. Y., WANG, P. G. Meristem shoot tip and bud cultures. In: EVANS, D. A. et al.Handbook of plant cell culture. Ne York: Macmillan. V. 1, P 177-227,1983.

KRIKORIAN, A. D. Medios de cultivo: generalidades, composicion y preparacion. In:ROCA, W. M.; MROGINKI, LA (eds). Cultivo de tejidos em Ia agricultura: fundamentos y aplicacions. Colombia : CIAT,1991. p 42-77.

LlNGAPPA, Y. Tissue cultures of solanum tuberosum and Ipomea pandurale. A ruer. J. BoI., n. 44, p.419-423, 1957.

MANGAT, B.S., PELEKIS, M.K. & CASSELS, A.C.- Changes in the starch content during organogenesis in vitro cultured Begonia rex stem explants Phisyologya Plantarum, n. 79. 1990. p.267-274.

MROGINSKI, L. A., ROCCA, W. M. - Establecimiento de cultivos de tejidos vegetales in vitro. In : ROCA, W. M. & MROGINKI, L. A. (eds). Cultivo de tejidos em Ia agricultura: fundamentos y aplicacions. Colombia: CIAT. 1991. p. 21 - 40.

MURASHIGE, T., SKOOG, F. A revised medi um for rapid growth and biossays with tobacco tissue cultures. Physiology Plantarum, V. 15. 1962. p. 473-497.

PARANHOS, J. T., ECHART, C. 1., FRANCO, E. T. H. Obtenção in vitro de brotos de Begonia rieger com thidiazuron, cinetina e acido naftaleno acético. Ciência Rural, v. 24, n. 3. 1994 p. 533-537.

PREVOT, P. Contribution histologie des phénomenes de néoformation chez Begonia rex Putz. Ver. Sci., n 5, 1948. p. 275-285.

RAMACHANDRA, S. & KHATAMIAN, H. Micropropagation of Peperomia and Begonia species using Petiole Segments. Hort Science, 24 (1). p.153-154. 1989.

STEWARD, F. C., NEWMANN, K. H., RAO, K. V. N. Investigations on the growth and metabolism of cultured explants of Daucus carota 11 effects of iron, molyodenum and manganese on metabolism Planta. n. 81, p. 351-371,1968.

SKOOG, F., MILLER, O. C. Chemical regulation of growth and organ formation in plant tissue cultured " in vitro ", Symp. Soco Exp. Biol., n. 11, p. 118-131, 1957.

TAKAYAMA, S. Begonia In: AMMIRATO, P. V. et aI. Handbook of plant cell culture. New York: Mc Graw-Will. 1990. V. 5. p. 253-283.

VAN OVERBEEK, J., CONLlN, M.E. & 8LAKESLEE, A. F. Factors in coconut milk essencial for growth and development of very young Datura embryos. Science, 94: 350-351,1941.

Published

1998-12-14

How to Cite

Franco, E. T. H., & Almeida, C. E. (1998). Ação de extratos vegetais e fitoreguladores na organogênese de Begonia rex. Ciência E Natura, 20(20), 131–142. https://doi.org/10.5902/2179460X26964