THE STREPTOMYCES POTENTIAL IN ISOLATED FROM MAUES REGION IN HYDROLYTIC ENZYMES PRODUCTION
Keywords:Hydrolytic enzymes, Streptomyces sp., Brazil nuts
The enzymes of microbial production area has grown in the last decades due to use in various industrial fields, and with that the research needs to innovations of existing products. The hydrolytic enzymes are biological catalysts of great commercial importance for low cost and high availability. This enzyme class has received special attention about his versatility in several areas of industrial biotechnology. The Brazil-nuts is a possible source of nutrients for the growth of micro-organisms in search of effective enzyme production and low cost. Were used two strains of Streptomyces sp. or production of protease, lipase, amylase and alkaline phosphatase using the residue Brazil-nut as carbon source in fermentation, semi-solid and liquid for the medium ISP-2 at pH 7.2 at a temperature 37°C for 15 days. The results showed that the best enzymatic activity was to protease secreted by Streptomyces sp. (1N) in semi solid fermentation. Concomitantly, was carried out an enzyme production kinetics with peak activity of 0.196 U / ml activity for 144 hours. The specific activity was observed a value of 2.17 U / mg protein for 240 hours. The production of enzymes excreted by Streptomyces sp. using the residue of Brazil-nuts is a resource for obtaining enzymes with high potential for solid state fermentation.
ANZAI, K. et al. Actinomycete bactéria isolated from the sediments at coastal and offshore area of Nagasaki prefecture, Japan: diversity and biological activity. Journal of Bioscience and Bioenginering. 2008, v.106, p. 215-217.
AWAD et al .Partial purification and characterization of extracellular protease from halophilic and thermotolerant strain Streptomyces pseudogrisiolus NC-15. Indian Journal of Biochemistry & Biophysics. 2013, v.50, p.305-31.
BRADFORD, M. M.; Anal. Biochem. 1976, 72, 248.
COLLA et al. Aplicações e produções de lipases microbianas. Revista de ciências extas aplicadas e tecnológicas. 2012, v.4, n.2, p.1-14.
CRUZ-JÚNIOR et al. Mulheres da floresta do vale do Guaporé e suas interações com o meio ambiente. Revista Estudos Feministas. 2012, v.18, n.3, p.913-925.
DAROIT D. J. et al Keratinolytic potential of a novel Bacillus sp.P45 isolated from the Amazon basin fish Piaractus mesopotamicus.Internation Biodeterioration and biodegradation. 2009, v.63, p.358-363.
DURAIPANDIYAN, V. et al. Antimicrobial properties of actinomycetes from the soil of Himalaya. Journal de Micologie Médicale. 2010, v.20, p. 15-20.
GAMA, E.V.G. et al. Produção de biomassa de erva-cidreira Lippia alba (Mill.) N.E.Br] sob adubação com composto de capim elefante inoculado e sem inoculação de actinomicetos. Revista Brasileira de Plantas Medicinais. 2012, v. 14, p. 163-168.
HASAN, F., SHAH, A. A., HAMEED, A. Industrial applications of microbial lipases. Enzyme and Microbial Technology. 2006, v. 39, n. 2, p. 235-251.
HOMMA et al. Extrativismo vegetal ou plantio: qual a opção para a Amazônia? Estudos avançados. 2012,v. 26, n. 74, p.167-186.
JAEGER, K.E.; DIJKSTRA, B.W.; REETZ, M.T. Bacterial Biocatalysts Molecular Biology, Three-Dimensional Structures, and Biotechonological Applications of Lipases. Annual Review Microbiology, v. 53, p. 315-351, 1999.
LI, S. et at. Technology prospecting on enzymes: application, marketing and engineering. Comput. Struct. Biotechnol. J. 2012, v.2, p. 1-1.
LOU, L. et al Recente advances in industrial applications of lipases and strategies for improving lipase properties. Jounal of bioprocess Engieering and Biorefinery. 2013, v.2, n.2, p.117-124.
MILLER, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugars, Analyical Chemistry. 1959 v.31, p. 426-428.
MOSTAFA et al. Optimization conditions of extracellular proteases production from a Newly isolated Streptomyces pseudogrisiolus NCR – 15. E-Journal of Chemistry. 2012, v. 9, n. 2, p. 949-961.
NIGAM, P.S. Microbial enzymes with special characteristics for biotechnological applications, Biomolecules. 2013, v.3, p.597-611.
PACHECO, A.M.; VILDES, Castanha-do-Brasil:da floresta tropical ao consumidor. Editorgraf, Florianópolis, M. S. 2006, p 176.
PACHECO DA ROSA, J. et al. Streptomyces lunalinharessi strain 235 shows the potential to inhibit bactéria involved in biocorrosion processes. BioMed Research International, 2013, v.2013, p.1-10.
PANDEY et al. New developments in solid-state fermentation. Bioprocesses and products. Process Biochem. 2000, v 35, p 1153–1169.
PILLAI, P.; MANDGE, S.; ARCHANA, G. Statistical optimization of production and tannery applications of a keratinolytic serine protease from Bacillus subtilis P13. Process Biochemistry. 2011, v. 46, n. 5, p. 1110- 1117.
RAJU et al .Binding Macrolide Polyketides from na Australian Marine-Derived Actinomycete Nocardiopsis sp. Chemistry European Journal. 2010, v.16, p.3194-3200.
How to Cite
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.