Antimicrobial and antiparasitic efficiency of desinfectants in the sanitation of vegetables in Natal city - RN

Authors

  • Ermeton Duarte do Nascimento Universidade Federal do Rio Grande do Norte Centro de Biociências Departamento de Microbiologia e Parasitologia
  • Feliphe Lacerda Souza Alencar Universidade Federal do Rio Grande do Norte

DOI:

https://doi.org/10.5902/2179460X12755

Keywords:

vegetables, sodium hypochlorite, acetic acid (vinegar), intestinal parasites and coliforms.

Abstract

http://dx.doi.org/10.5902/2179460X12755

This study evaluated the efficiency of sodium hypochlorite (1%) and the acetic acid (vinegar) (6.6%) in the sanitation of vegetables (lettuce, cabbage, cilantro and kale) from six establishments, subdivided into three supermarkets and three fairs, located in the city of Natal - RN, during the period from June  to October, 2013. The samples were analyzed in order to describe the presence of contaminants and then were submitted to treatment with sanitizer solutions. For parasitological analysis, we used the methods of Hoffman, Pons and Janer (HPJ) and Faust. For bacterial analysis, we used the technique of chromogenic substrate and subsequent quantification of coliform groups by the most probable number (MPN). For statistical analysis, in addition to descriptive statistics, considering an α=0.05, the Student t test was used. In total of 24 samples analyzed, in 41,7% were found parasitic forms and the most prevalent ones was the Endolimax nana (22.2%). Among the samples analyzed, 100% were contaminated with total coliforms and 83,3% with thermotolerant coliforms. The lettuce had the highest rate of contamination by parasites (50%), among the samples from the control group, as well as, the highest average of contamination by total coliforms and thermotolerants coliforms, above 2400 NMP/100mL and 1356 NMP/100mL, respectively. The vegetables coming from the fairs had the highest prevalence of contamination by parasites (70%), total coliforms (53.7%) and thermotolerants coliforms (56.8%).However, this study showed there is no difference between the risk of a vegetable bought in a supermarket or a fair being contaminated by parasites (p ≤ 0.223), total coliforms (p ≤ 0.193) and/or thermotolerants coliforms (p ≤ 0.789). The solution of sodium hypochlorite (1%) was more efficient to contaminated samples by parasites (p ≤ 0.001), total coliforms (p ≤ 0.000) and thermotolerants coliforms (p ≤ 0.001). It is concluded that vegetables can be great reservoirs of pathogenic parasites and coliform groups, making necessary pre-emptive measures aimed at sanitary quality of water used for irrigation of vegetables and the proper disinfection of these foods.

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Author Biographies

Ermeton Duarte do Nascimento, Universidade Federal do Rio Grande do Norte Centro de Biociências Departamento de Microbiologia e Parasitologia

Professor Assistente III da UFRN, é graduado em C. Biológicas e Biomedicina, tem especialização em Microbiologia, mestrado em C. Biológicas e Doutorado em Desenvolvimento e Meio Ambiente/UFRN (em andamento). Sua linha de pesquisa é em Microbiologia médica e ambiental, com foco em resistência bacteriana aos antimicrobianos.

Feliphe Lacerda Souza Alencar, Universidade Federal do Rio Grande do Norte

Bacharel em Biomedicina pela UFRN, mestrando do Programa de Desenvolvimento e Meio Ambiente - PRODEMA/UFRN, Com linha de pesquisa em microbiologia ambiental e médica.

Published

2014-05-14

How to Cite

Nascimento, E. D. do, & Alencar, F. L. S. (2014). Antimicrobial and antiparasitic efficiency of desinfectants in the sanitation of vegetables in Natal city - RN. Ciência E Natura, 36(2), 92–106. https://doi.org/10.5902/2179460X12755

Issue

Section

Biology