Laurus Nobilis L. essential oil potentializes the activity of antibiotics against multirresistant bacteria
DOI:
https://doi.org/10.5902/2236583483845Keywords:
Antibacterials, Escherichia coli, Essential oil, Laurus, Staphylococcus aureusAbstract
Objective: The aim of this study was to evaluate the antibacterial and combined antibiotic activity of Laurus nobilis essential oil. Methods: The essential oil was obtained by hydrodistillation with a yield of 1%. Triplicate tests were carried out using the gas contact method to evaluate the antibacterial activity against multi-resistant strains of Escherichia coli 27 and Staphylococcus aureus 358. Petri dishes were filled with Brain Heart Infusion (BHI), the seeded bacteria and a sterile paper disk in the center. 100% L. nobilis essential oil was placed on the lid and incubated in an oven at 37°C for 24 hours, where the inhibition halo was observed. In the modulation test, antibiotic discs (amikacin, gentamicin, ciprofloxacin and norfloxacin) were placed in the center of the plates. After this procedure, the plate was inverted and 10 μL of oil was placed on the lids, allowing volatilization and interaction with the antibiotic discs. Results: In the direct activity it was seen that bacterial growth was not inhibited, i.e. the oil at a concentration of 10µL did not exhibit antibacterial activity. When the oil was associated with antibiotics, there was a modifying effect on the action of the antibiotics for both strains. Against S. aureus 358 there was a noticeable increase in the halo for all the antimicrobials used, with ciprofloxacin standing out as having a greater interaction with the oil. For E. coli 27 there was a 20% increase in the halo for each antimicrobial used. Conclusion: The essential oil of L. nobilis did not have a direct effect on the strains tested, but it was able to modulate the activity of all the antibiotics used for the two strains, demonstrating a significant combined effect.
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