Validation and application of a chromatographic method for evaluation of commercial vegetable oils possibly adulterated

Authors

  • Katarynna Santos Araújo Centro de Tecnologias Estratégicas do Nordeste, Recife, PE
  • Mariana Oliveira Barbosa Centro de Tecnologias Estratégicas do Nordeste, Recife, PE
  • Carolina Barbosa Malafaia Centro de Tecnologias Estratégicas do Nordeste, Recife, PE https://orcid.org/0000-0001-6482-5341
  • Daniella Carla Napoleão Centro de Tecnologias Estratégicas do Nordeste, Recife, PE https://orcid.org/0000-0002-2760-562X

DOI:

https://doi.org/10.5902/2236117035071

Keywords:

Adulteration, GC-FID, olive oil, soybean oil, unsaturated fatty acids

Abstract

A method of separation, identification and quantification of fatty acid methyl esters (FAMEs) was developed by gas chromatography with flame ionization detector (GC-FID) using a basic transesterification. In this sense, there were analyzed FAMEs in commercial samples of vegetable oils from soybean and olive oil. The referred method was linear (r>0.99), accurate and precise for palmitic (C16:0), linoleic (C18:2), oleic (C18:2), linolenic (C18:3) and stearic (C18:0) acids. The limits of detection (LOD) and quantification (LOQ) were from 0.03 to 0.31 and 0.08 to 0.94 mg.mL-1 for the five fatty acids, respectively. The results demonstrated that the unsaturated fatty acids were the most abundant for the two samples, being the oleic acid (C18:1) the major in three brands of olive oil (D, E and F), and the linoleic acid (C18:2) the most abundant in soybean oil and the other brands of olive oil (G, H and I), suggesting a possible adulteration in these brands. The proposed method could be considered a tool for the investigation of adulteration in commercial vegetable oils for guaranteed reliability in the results to be comparable with correlated legislations.

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Author Biographies

Katarynna Santos Araújo, Centro de Tecnologias Estratégicas do Nordeste, Recife, PE

Bacharel em Química Industrial pela Universidade Federal de Pernambuco

Mariana Oliveira Barbosa, Centro de Tecnologias Estratégicas do Nordeste, Recife, PE

Especialização em Residência em Saùde da Família e das Comunidades. (Carga Horária: 1920h).
UNASUS - UFPE

Carolina Barbosa Malafaia, Centro de Tecnologias Estratégicas do Nordeste, Recife, PE

Doutorado em Ciências Biológicas (Conceito CAPES 5).
Universidade Federal de Pernambuco, UFPE, Brasil.

Daniella Carla Napoleão, Centro de Tecnologias Estratégicas do Nordeste, Recife, PE

Professora Adjunta AII da Universidade Federal de Pernambuco

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Published

2018-12-19

How to Cite

Araújo, K. S., Barbosa, M. O., Malafaia, C. B., & Napoleão, D. C. (2018). Validation and application of a chromatographic method for evaluation of commercial vegetable oils possibly adulterated. Revista Eletrônica Em Gestão, Educação E Tecnologia Ambiental, 22, e13. https://doi.org/10.5902/2236117035071

Issue

Section

ENVIRONMENTAL THECNOLOGY

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