Multiplication and <i>in vitro</i> rooting of <i>Handroanthus impetiginosus</i> (Mart. Ex DC.) Mattos
DOI:
https://doi.org/10.5902/1980509827012Parole chiave:
In vitro propagation, Woody plant, Plant tissue culture, Ipê-roxoAbstract
This study aimed to evaluate cytokinin influence during in vitro multiplication stage and activated charcoal in combination with indole-3-butyric acid (IBA) during in vitro rooting of Handroanthus impetiginosus. For in vitro multiplication, nodal segments of shoots obtained from in vitro seedlings establishment were inoculated in Woody Plant Medium (WPM), with and without the following concentrations of 6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ): 0.5; 1.0; 2.0; 4.0 and 8.0 µmol L-1. For in vitro rooting, shoots were inoculated in WPM containing combinations among IBA (1.0; 3.0; 6.0 and 9.0 μmol L-1) and activated charcoal (1.0; 2.0 and 3.0 g L-1), as well as their absence within culture medium. The highest number of shoots and buds was reached after using TDZ 8.0 μmol L-1, where in the best rooting occurred after adding activated charcoal 2.0 g L-1, regardless of IBA concentration. Our results show that using TDZ in a suitable level for in vitro multiplication stage followed by using 2.0 g L-1 of activated charcoal to obtain well rooted shoots is efficient for ipê-roxo micropropagation in a low cost-manner and quickly, besides providing knowledge about how to keep this threatened extinction species in an in vitro environment, which may help other conservation studies.
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Riferimenti bibliografici
BANERJEE, A.; BANDYOPADHYAY, S.; RAYCHAUDHURI, S. S. In vitro regeneration of Hypericum perforatum L. using thidiazuron and analysis of genetic stability of regenerants. Indian Journal of Biotechnology, New Delhi, v. 11, n. 1, p. 92-98, 2012.
BAUL, T. K.; MEZBAHUDDIN, M.; MOHIUDDIN, M. Vegetative propagation and initial growth performance of Stereospermum suaveolens DC., a wild tropical tree species of medicinal value. New Forests, Dordrecht, v. 37, n. 3, p. 275-283, 2009.
BERTONI, G. Indole butyric acid-derived auxin and plant development. Plant Cell, Waterbury, v. 23, n. 3, p. 845, 2011.
BRONDANI, G. E. et al. Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden. Revista Árvore, Viçosa, v. 33, n. 1, p. 11-19, 2009.
FERREIRA, D. F. Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, Lavras, v. 38, n. 2, p. 109-112, 2014.
FIORITO, S. et al. Growth inhibitory activity for cancer cell lines of lapachol and its natural and semi-synthetic derivatives. Bioorganic & Medicinal Chemistry Letters, Amsterdã, v. 24, n. 2, p. 454-457, 2014.
GANTAIT, S.; MANDAL, N.; DAS, P. K. Impact of auxins and activated charcoal on in vitro rooting of Dendrobium chrysotoxum Lindl. cv. Golden Boy. Journal of Tropical Agriculture, New Delhi,v. 47, n. 1-2, p. 84-86, 2009.
GIGLI-BISCEGLIA, N. et al. Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner. Development, Cambridge, v. 145, p. 1-14, 2018.
GIRI, D.; TAMTA, S. Effect of plant growth regulators (PGRs) on micropropagation of a vulnerable and high value medicinal plant Hedychium spicatum. African Journal of Biotechnology, Nairóbi, v. 10, n. 20, p. 4040-4045, 2011.
GRATTAPAGLIA, D.; MACHADO, M. A. Micropropagação. In: TORRES, A.C.; CALDAS, L.S.; BUSO, J.A. Cultura de tecidos e transformação genética de plantas. Brasília: SPI/Embrapa-CNPH, v.1. 1998. p.183-260.
GROSE, S. O.; OLMSTEAD, R. G. Taxonomic revisions in the polyphyletic genus Tabebuia s. L. (Bignoniaceae). Systematic Botany, Laramie, v. 32, n. 3, p. 660-670, 2007.
HOLUBOVÁ, K. et al. Modification of barley plant productivity through regulation of cytokinin content by reverse-genetics approaches. Frontiers in Plant Science, Lausanne, v. 9, art. 1676, p. 1-18, 2018.
HUSSAIN, H.; GREEN, I. R. Lapachol and lapachone analogs: a journey of two decades of patent research (1997-2016). Expert Opinion on Therapeutics Patents, Oxfordshire, v. 27, n. 10, p. 1111-1121, 2017.
INOCENTE, V. H. H.; NIENOW, A. A.; TER, L. Time of treatment with IBA in Olive cultivars rooting. Revista Brasileira de Fruticultura, Jaboticabal, v. 40, n. 1, p. 1-6, 2018.
KATARIA, S.; GURUPRASAD, K. N. Interaction of cytokinins with UV-B (280-315 nm) on the expansion growth of cucumber cotyledons. Horticulture International Journal, Edmond, v. 2, n. 2, p. 46-54, 2018.
LARRABURU, E. E.; APÓSTOLO, N. M.; LLORENTE, B. E.In Vitro propagation of pink lapacho: response surface methodology and factorial analysis for optimisation of medium components. International Journal of Forestry Research, London, v. 2012, n. 2012, p 1-9, 2012.
LEITZKE, L. N.; DAMIANI, C. R.; SCHUCH, M. W. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira. Ciência e Agrotecnologia, Lavras, v. 34, n. 2, p. 352-360, 2010.
LLOYD, G.; McCOWN, B. Commercially feasible micropropagation of montain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings of the International Plant Propagator's Society, Bellefonte, v. 30, n. 5, p. 421-327, 1981.
LORENZI, H. Árvores brasileiras: Manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 4. ed. Nova Odessa: Editora Plantarum, 2002. v. 1, p.66.
MARTINS, J. P. R. et al. Crescimento e aspectos sintomatológicos na aclimatização de Ipê-roxo. Cerne, Lavras, v. 17, n. 4, p. 435-442, 2011.
MAZRI, M. A. Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm (Phoenix dactylifera L.) cv. Boufeggous. Journal of Plant Biochemistry and Biotechnology, Basel, v. 24, n. 3, p. 268-275, 2015.
NISLER, J. et al. Novel thidiazuron-derived inhibitors of cytokinin oxidase/dehydrogenase. Plant Molecular Biology, Basel, v. 92, n. 1-2, p. 235-248, 2016.
OLIVEIRA, A. K. M.; SCHELEDER, E. J. D.; FAVERO, S. Caracterização morfológica, viabilidade e vigor de sementes de Tabebuia chrysotricha (Mart. ex. DC.) Standl. Revista Árvore, Viçosa, v. 32, n. 6, p. 1011-1018, 2008.
OLIVEIRA, T. P. F. et al. Efeito do ácido indol-3-butírico no enraizamento de miniestacas de Ipê-roxo (Handroanthus heptaphyllus MATTOS). Ciência Florestal, Santa Maria, v. 25, n. 4, p. 1043-1051, 2015.
SÁENZ, L. et al. Influence of form of activated charcoal on embryogenic callus formation in coconut (Cocos nucifera). Plant Cell, Tissue and Organ Culture, Dordrecht, v. 100, n. 3, p. 301-308, 2010.
SEKELI, R. et al. Better rooting procedure to enhance survival rate of field grown Malaysian Eksotika Papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene. ISRN Biotechnology, New York, v. 2013, art. ID 958945, p. 1-10, 2013.
SIQUEIRA, A. C. M. F. et al. Relatório sobre conservação de recursos genéticos de essências nativas. Revista do Instituto Florestal, São Paulo, SP. 47 p., 1992.
SOARES, F. P. et al. Taxa de multiplicação e efeito residual de diferentes fontes de citocininas no cultivo in vitro de Hancornia speciosa Gomes. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 152-157, 2011.
VASCONCELOS, A. G. V. et al. Hiperidricidade: uma desordem metabólica. Ciência Rural, Santa Maria, v. 42, n. 5, p. 837-844, 2012.
VIEITEZ, A. M. et al. In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 98, n. 2, p. 135-145, 2009.
WYBOUW, B.; RYBEL, B. D. Cytokinin – A Developing Story. Trends in Plant Science, Cambridge, v. 24, n. 2, p. 177-185, 2019.
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