INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i>
DOI:
https://doi.org/10.5902/1980509814572Keywords:
regeneration, woody species, plant growth regulators, culture mediumAbstract
http://dx.doi.org/10.5902/1980509814572
The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of Eucalyptus benthamii x Eucalyptus dunnii. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures.
Downloads
References
ALCANTARA, G. B.; BESPALHOK FILHO, J. C.; QUOIRIN, M. Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Scientia Agrícola, Piracicaba, v. 68, n. 2, p. 246-251, 2011.
ALVES, E. C. S. C.; XAVIER, A.; OTONI, W. C. Organogênese de explante foliar de clones de Eucalyptus grandis x Eucalyptus urophylla. Pesquisa Agropecuária Brasileira, Brasília, v. 39, n. 5, p. 421-430, 2004a.
ALVES, E. C. S. C.; XAVIER, A.; OTONI, W. C. Organogênese in vitro a partir de explante caulinar na regeneração de clones de Eucalyptus grandis W. Hill ex Maiden x Eucalyptus urophylla S. T. Blake. Revista Árvore, Viçosa, v. 28, n. 5, p. 643-653, 2004b.
ANDRADE, W. F.; ALMEIDA, M.; GONÇALVES, A. N. Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina. Pesquisa Agropecuária Brasileira, Brasília, v. 41, n. 12, p. 1715-1719, 2006.
AZMI, A. et al. Bud regeneration from Eucalyptus globulus clones and seedlings through hormonal imbalances induced by Agrobacterium tumefaciens strain. Plant Science, v. 127, n. 1, p. 81-90, 1997.
BARRUETO CID, L. P. et al. Plant regeneration from seedling explants of Eucalyptus grandis x Eucalyptus urophylla. Plant Cell Tissue and Organ Culture, Netherlands, v. 56, n. 1, p. 17-23, 1999.
BISHT, P.; SHARMA V. K.; JOSHI I.; KAPOOR M. L. Micropropagation of newly produced F1 hybrid of Eucalyptus (E. tereticornis Sm. x E. camaldulensis Dehn. Southern Form). Silvae Genetica, Frankfurt, v. 48, n. 2, p. 104-108. 1999.
BORGES, S. R. et al. Multiplicação in vitro de clones híbridos de Eucalyptus globulus. Revista Árvore, Viçosa, v. 35, n. 2, p. 173-182, 2011.
BRONDANI, G. E. et al. Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage X Eucalyptus dunnii Maiden, Revista Árvore, Viçosa, v. 33, n. 1, p. 11-19, 2009.
BRONDANI, G. E.; DUTRA, L. F.; WENDLING, I.; GROSSI, F.; HANSEL, F. A.; ARAUJO, M. A. Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii). Acta Scientiarum. Agronomy. Maringá, v. 33, n. 4, p. 655-663, 2011.
CHANG, S. H. et al. Thidiazuron enhancement of plant regeneration from leaf calli of superior clones of Eucalyptus camaldulensis. Journal Forestry Sciences, v. 15, p. 81-90, 2000.
CORREIA D. et al. Efeito do meio de cultura líquido e sólido no crescimento e desenvolvimento de gemas de Eucalyptus grandis x Eucalyptus urophylla na multiplicação in vitro. IPEF, Piracicaba, n. 48/49, p. 107-116, 1995.
DEL PONTE, E. M. et al. Multiplicação e enraizamento in vitro de Eucalyptus globulus subsp. globulus Labill. Revista Árvore, Viçosa, v. 25, p. 1-8, 2001.
DIBAX, R. et al. Plant Regeneration from cotyledonary explants of Eucalyptus camaldulensis Dehn and histological study of organogenesis in vitro. Brazilian Archives of Biology and Technology, Curitiba, v. 53, n. 2, p. 311-318, 2010a.
DIBAX, R. et al. Organogenesis and Agrobacterium tumefaciens mediated transformation of Eucalyptus saligna with P5CS gene. Biologia Plantarum, v. 54, n. 1, p. 6-12, 2010b.
GOMES, F.; CANHOTO, J. M. Micropropagation of Eucalyptus nitens Maiden (Shining Gum). In Vitro Cellular and Developmental Biology-Plant, v. 39, p. 316-321, 2003.
GLOCKE, P. et al. Micropropagation of juvenile tissue of Eucalyptus erythronema x Eucalyptus stricklandii cv. “Urrbrae Gem”. In Vitro Cellular and Developmental Biology-Plant, v. 42, p. 139-143, 2006.
KIM, J. Y. et al. Two polyphenol oxidases are differentially expressed during vegetative and reproductive development and in response to wounding in the Fuji apple. Plant Science, v. 161, n. 6, p. 1145–1152, 2001.
LLOYD, G.; MCCOWN, B. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. International Plant Propagation Society Proceedings, v. 30, p. 421-427, 1980.
MACHADO, L. O. R. et al. Transformation and regeneration studies of elite eucalypt hybrids: optimisation of physical and biological parameters. Proceedings of IUFRO Conference on Silviculture and Improvement of Eucalypts, v. 2, p. 192-199, 1997.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, Copenhagem, v. 15, n. 3, p. 473-497, 1962.
NETTO, A. B. P. et al. Shooting control in Eucalyptus grandis x Eucalyptus urophylla hybrid: comparative effects of 28-homocastasterone and a 5a-monofluoro derivative. Plant Cell, Tissue and Organ Culture, Netherlands, v. 86, n. 3, p. 329-335, 2006.
NICCOL, R. J. ; REGAN, P. A ; DE FILIPPIS, L. F. Simplified protocol for the micropropagation of selected Eucalyptus and Banksia species. Australian Forestry, v. 57, n. 4, p. 143-147, 1994.
NUGENT, G. et al. Adventitious bud induction in Eucalyptus globulus Labill. In Vitro Cellular and Developmental Biology - Plant, v. 37, n. 3, p. 388-391, 2001.
PALUDZYSZYN FILHO, E.; SANTOS, P. E. T. dos; FERREIRA, C. A. Eucaliptos indicados para plantio no Estado do Paraná. Colombo: Embrapa Florestas – CNPF 2006. (Documentos; 129).
QUISEN, R. C. et al. Selective agents and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis cotiledonary culture. Brazilian Archives of Biology and Technology, Curitiba, v. 52, n. 6, p. 1485-1492, 2009.
SANKARA-RAO, K. In vitro meristem cloning of Eucalyptus tereticornis Sm. Plant Cell Reports, v. 7, p. 546-549, 1988.
SILVA, F. de A. S.; AZEVEDO, C. A. V. de. Principal Components Analysis in the Software Assistat-Statistical Attendance. In: WORLD CONGRESS ON COMPUTERS IN AGRICULTURE, 7., 2009, Reno-NV-Proceedings… American Society of Agricultural and Biological Engineers, 2009.
TOURNIER, V. et al. An efficient procedure to stably introduce genes into an economically important pulp tree (Eucalyptus grandis x Eucalyptus urophylla). Transgenic Research, v. 12, n. 4, p. 403-411, 2003.
TRINDADE, H.; FERREIRA, J. G.; PAIS, M. S. ALONI, R. The role os cytokinin and auxin in rapid multiplication of shoots of Eucalyptus globulus grown in vitro. Australian Forestry, v. 53, n. 3, p. 221-223, 1990.
WERNER, E. T. et al. Controle da calogênese do pau-brasil in vitro. Revista Árvore, Viçosa, v. 33, n. 6, p. 987-996, 2009.
