Evaluation of the hydrolysis yield of banana tree pseudostem using Penicillium oxalicum enzyme extract
DOI:
https://doi.org/10.5902/2179460X84172Keywords:
Banana pseudostem, Crude enzyme extract, Hydrolysis, EthanolAbstract
The objective of this research was to perform, under different conditions, the hydrolysis of banana pseudostem using enzymes of the species Penicillium oxalicum. A hydrothermal pretreatment (121 °C/30 min) was performed, and subsequently the characterization of the biomass in natura and pretreated was used as a substrate in the production of enzymatic extracts. The enzymatic activities were determined (CMCase, Avicelase, and FPase), showing better results for the extract produced in biomass in natura. Biomass hydrolysis was performed under different conditions, using the crude enzyme extract produced in pretreated biomass. Through a Rotational Central Composite Design (RCCD), the best hydrolysis condition (20 mg of biomass, 2 mL of enzyme, and pH 5) occurred in assay 9, obtaining 26.36% of hydrolysis yield. Under the same conditions, another hydrolysis was performed, using the enzyme extract produced from in natura biomass, resulting in a 42% hydrolysis yield. It was found that the pre-heat treatment used did not positively influence the hydrolysis process of this biomass. The P. oxalicum strain (F-3380) showed potential to produce cellulolytic enzymes, and the banana pseudostem in natura is an attractive biomass for ethanol production.
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