Spectrophotometric method for L (+) Arginin, L (-) Asparagin and L (+) Lysin determination in pharmaceutical drugs, with o-diacetylbenzene
DOI:
https://doi.org/10.5902/2179460X24928Abstract
In this work the main analytical parameters for a spectrophotometric method of amino-acid analysis were determined.
The Ringbom curve for L (+) Arginin (550 nm), L (-) Asparing (547 mn) and L (+) Lysin (547 mn) determination with o-diacetylbenzene showed different values for concentrations.
The best rang of concentration was: L (+) Arginin 150 to 500µg/ml with a relative error of 5.26%; L (-) Asparagin 40 to 200µg/ml with relative error 1.43%; L (+) Lysin 8 to 44µg/ml with a relative error of 0.18%.
Among the different compounds studied, it was observed the absorptions of the tiamin cloride (Vitamin B1), riboflavin choride (Vitamin B2), piridoxin cloride (Vitamin B6), carnitin, buclisin, folic acid and nicotinamide and that this compound in presence of o-diacetylbenzene do not interfere with the absorptions of the amino-acids L (+) Arginin, L (-) Asparagin and L (+) Lysin during their determination. On the other hand, the absorption of the compounds cianocobalamin and ɤ aminbutiric acid or the absorptions of these compounds in presence of o-diacetylbenzene interfere with absorptions of the aminoacids studied.
The absorption spectra of these three pharmaceutical drugs was obtained with o-diacetylbenzene and we found out the possibility of their direct determination by the method described.
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SANTOS, A.C.; SCHENKEl, W.F.; LOPES, W.; VINADÉ, M.E.C.; BERNEIRA, Z.C .. Rev. Centro Ciências da Saúde, 7 (1 e.2):97-120,1979.
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