Influence of Atta sexdens rubropilosa on seeds germination of Copaifera langsdorffii
DOI:
https://doi.org/10.5902/2179460X67030Keywords:
Overcome dormancy, Saúva-limão, Pau-d'óleo, CoumarinsAbstract
Considering the information gaps on the effect of ant action on germination, this study aimed to evaluate the influence of ants of the species Atta sexdens rubropilosa in overcoming seed dormancy of Copaifera langsdorffii comparing the results obtained with traditional methodologies, in addition to evaluating the presence of coumarin in the seeds. For each germination treatment (7) 100 seeds were used, with 4 replications: (1) control, (2) seeds without aril, (3) seeds with immersion in acid for 5 minutes, (4) seeds with immersion in acid for 10 minutes, (5) sanded seeds, (6) collected seeds with presence of fissures on the forehead and, (7) seeds with partial cotyledons. The seeds were maintained at a temperature of 25 °C, being evaluated the germination and vigor. Data were subjected to analysis of variance and means were compared by the Tukey test at 5% probability. The best germination was obtained in treatment 6 (seeds with fissures caused by ants), which also provided the best germination speed index and the shortest germination time. The chemical analysis of the seeds also indicated the presence of coumarins in the aril and in the external structure (forehead) of the seeds, demonstrating the presence of germination inhibitors, indicating the importance of this ant species in the overcome dormancy process of seeds of Copaifera langsdorffii.
Downloads
References
ANDREANI JUNIOR, R.; MELLO, W. S.; SANTOS, S. R. G.; KOZUSNY-ANDREANI, D. I. Superação da dormência de sementes de três essências florestais nativas. Revista da Universidade Vale do Rio Verde, Três Corações, v. 12, n. 1, p. 470-479, 2014. http://dx.doi.org/10.5892/ruvrd.v12i1.1390.
ARRUDA, D. M.; BRANDÃO, D. O.; VELOSO, M. D. M.; NUNES, Y. R. F. Germinação de três espécies de Fabaceae típicas de floresta estacional decidual. Pesquisa Florestal Brasileira, Colombo, v. 35, n. 82, p. 135-142, 2015. http://dx.doi.org/10.4336/2015.pfb.35.82.672.
BEWLEY, J. D.; BRADFORD, K. J.; HILHORST, H.; NONOGAKI, H. Physiology of development, germination and dormancy. 3rd ed. New York: Springer, 2013.
BRADFORD, K. J.; NONOGAKI, H. Seed development, dormancy and germination. Oxford: Blackwell, 2007.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília: Secretaria de Defesa Agropecuária. Mapa/ACS, 2009.
CARVALHO, P. E. R. Espécies arbóreas brasileiras. Vol. 1. 1ed. Brasília: Embrapa Informação Tecnológica, 2003.
CARVALHO, K. S.; BALCH, J.; MOUTINHO, P. Influências de Atta spp. (Hymenoptera: Formicidae) na recuperação da vegetação pós-fogo em floresta de transição amazônica. Acta Amazonica, Manaus, v. 42, n. 1, p. 81-88, 2012. http://dx.doi.org/10.1590/S0044-59672012000100010.
CORRÊA, M. M. et al. How leaf-cutting ants impact forests: drastic nest effects on light environment and plant assemblages. Oecologia, Berlin, v. 162, p. 103-115, 2010. http://dx.doi.org/10.1007/s00442-009-1436-4
COSTA, T. S.; AOYAMA, E. M.; FALQUETO, A. R. Superação de dormência e salinidade na germinação de sementes e vigor de plântulas de Canavalia rosea (Sw.) DC. – Fabaceae. Revista de Ciências Agro-Ambientais, Alta Floresta, v. 11, n. 1, p. 77-87, 2013.
EIRA, M. T. S.; MARTINS NETTO, D. A. Germinação e conservação de sementes de espécies lenhosas. In: RIBEIRO, J. F. (Ed.) Cerrado: Matas de Galeria. Planaltina: Embrapa-CPAC, 1998. p. 97-117.
GUERRA, M. E. C.; MEDEIROS FILHO, S.; GALLÃO, M. I. Morfologia de sementes, de plântulas e da germinação de Copaifera langsdorffii Desf. (Leguminosae - Caesalpinioideae). Cerne, Viçosa, v. 12, n. 4, p. 322-328, 2006.
LABOURIAU, L. G. A germinação das sementes. Washington: Secretaria Geral da Organização dos Estados Americanos – OEA, 1983.
LABOURIAU, L. G.; VALADARES, M. E. B. On the germination of seeds of Calotropis procera (Ait.) Ait. f. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 48, n. 2, p. 263-284, 1976.
LEAL, R. I.; OLIVEIRA, P. S. Interactions between fungus-frowing ants (Attini), fruits and seeds in Cerrado vegetation in Southeast Brazil. Biotropica, Saint Louis, v. 30, n. 2, p. 170-178, 1998.
LEITE, R. R.; ARAÚJO, S. S. C.; OLIVEIRA, E. G. Remoção dos frutos de Miconia albicans (SW.) Triana (Melastomataceae) por formigas na borda e no interior de um fragmento de Cerrado, Curvelo, MG. Revista Árvore, Viçosa, v. 37, n. 3, p. 469-478, 2013. http://dx.doi.org/10.1590/S0100-67622013000300010.
MAGUIRE, J. D. Speed of germination aid in selection and evaluation for seedling and vigor. Crop Science, Madison, v. 2, n. 2, p. 176-177, 1962.
MATOS, F. J. A. Introdução a Fitoquímica Experimental. 2ed. Fortaleza: UFC, 2009.
MEYER, S. T.; LEAL, I. R.; WIRTH, R. Persisting hyper-abundance of leaf-cutting ants (Atta spp.) at the edge of an old Atlantic Forest fragment. Biotropica, Saint Louis, v. 41, p. 711-716, 2009. http://dx.doi.org/10.1111/j.1744-7429.2009.00531.x
MEYER, S. T.; LEAL, I. R.; TABARELLI, M.; WIRTH, R. Ecosystem engineering by leafcutting ants: nests of Atta cephalotes drastically alter forest structure and microclimate. Ecological Entomology, London, v. 36, p. 14-24, 2011. http://dx.doi.org/10.1111/j.1365-2311.2010.01241.x
MEYER, S. T. et al. Leafcutting ants as ecosystem engineers: topsoil and litter perturbations around Atta cephalotes nests reduce nutrient availability. Ecological Entomology, London, v. 38, p. 497-504, 2013. http://dx.doi.org/10.1111/een.12043
OLIVEIRA, A. K. M. et al. Superação de dormência em sementes de Parkia gigantocarpa (Fabaceae-Mimosidae). Ciência Florestal, Santa Maria, v. 22, n. 3, p. 533-540, 2012. http://dx.doi.org/10.5902/198050986620.
PEREIRA, R. S.; SANTANA, D. G.; RANAL, M. A. Emergência de plântulas oriundas de sementes recém-colhidas e armazenadas de Copaifera langsdorffii Desf. (Caesalpinioideae), Triângulo Mineiro, Brasil. Revista Árvore, Viçosa, v. 33, n. 4, p. 643-652, 2009. http://dx.doi.org/10.1590/S0100-67622009000400007.
PIÑA-RODRIGUES, F. C. M.; AGUIAR, I. B. Maturação e dispersão de sementes. In: AGUIAR, I. B.; PIÑA-RODRIGUES, F. C. M.; FIGLIOLIA, M. B. (Eds.) Sementes florestais tropicais. Brasília: ABRATES, 1993. p. 215-274.
RODRIGUES, A. P. D.; OLIVEIRA, A. K. M.; LAURA, V. A.; YAMAMOTO, C. R.; CHERMOUTH, K. S.; FREITAS M. H. F. Tratamentos para superação de dormência de sementes de Adenanthera pavovina L. Revista Árvore, Viçosa, v. 33, n. 4, p. 617-623, 2009. https://doi.org/10.1590/S0100-67622009000400004
ROSSATTO, D. R.; KOLB, R. M. Germinação de Pyrostegia venusta (Bignoniaceae), viabilidade de sementes e desenvolvimento pós-seminal. Brazilian Journal of Botany, São Paulo, v. 33, n. 1, p. 51-60, 2010. http://dx.doi.org/10.1590/S0100-84042010000100006.
SALOMÃO N. A. et al. Germinação de sementes e produção de mudas e plantas do Cerrado. Brasília: Rede de Sementes do Cerrado, 2003.
SANTOS, B. A. et al. Drastic erosion in functional attributes of tree assemblages in Atlantic forest fragments of northeastern Brazil. Biological Conservation, Oxford, v. 141, n. 1, p. 249-260, 2018. https//doi.org/10.1016/j.biocon.2007.09.018.
SANTOS, M. A.; BRAGA, L. F.; RONDON NETO, R. M.; SORATO, A. M. C. Aspectos morfológicos e fisiológicos da germinação e morfometria de frutos e sementes de Swartzia recurva Poep. (Fabaceae). Ciência e Natura, Santa Maria, v. 37, n. 4, p. 34-54, 2015. http://dx.doi.org/105902/2179460X17309.
SILVA, A.; SOUZA, D. J. Interação entre Atta sexdens e espécie arbórea Copaifera langsdorffii Desf. em remanescente florestal de cerrado. Revista Verde de Agroecologia e Desenvolvimento Sustentável, v. 9, n. 3, p. 182-189, 2014.
SIMÕES, C. M. O. et al. Farmacognosia: do produto natural ao medicamento. Porto Alegre/Florianópolis: Artmed Editora, 2017.
SOLOMON, S. E. et al. Paleodistributions and comparative molecular phylogeography of leafcutter ants (Atta spp.) provide new insight into the origins of Amazonian diversity. PLOS ONE, California, v. 3, n. 7, p. e2738, 2008. https//doi.org/10.1371/journal.pone.0002738.
SOUZA, M. L.; SILVA, D. R. P.; FANTECELLE, L. B.; LEMOS FILHO, J. P. Key factors affecting seed germination of Copaifera langsdorffii, a Neotropical tree. Acta Botanica Brasilica, Belo Horizonte, v. 29, n. 4, p. 473-477, 2015. http://dx.doi.org/10.1590/0102-33062015abb0084.
SOUZA, M. L.; FAGUNDES, M. Seed predation of Copaifera langsdorffii (Fabaceae): a tropical tree with supra-annual fruiting. Plant Species Biology, Sapporo, v. 32, n. 1, p. 66-73, 2017. https//doi.org/10.1111/1442-1984.12128.
SOUZA, L. M. S.; SILVA, J. B.; GOMES, N. S. B. Qualidade sanitária e germinação de sementes de copaíba. Bioscience Journal, Uberlândia, v. 29(Supplement 1), p. 1524-1531, 2013.
SOUZA, R. M.; ANJOS, N.; CORDEIRO, G.; MOURÃO, S. A. Primeiro registro de Atta sexdens rubropilosa Forel atacando árvores de Nim, Azadirachta indica A. Juss. Arquivos do Instituto de Biologia, São Paulo, v. 76, n. 4, p. 729-733, 2009. https//doi.org/10.1590/1808-1657000722015
TAVARES, P. R. A.; ALVES-JUNIOR, V. V.; MORAIS, G. A. Does Atta laevigata (Smith, 1858) act as Solanum lycocarpum seed dispersers? Sociobiology, Feira de Santana, v. 63, n. 1, p. 682-687, 2016. https://doi.org/10.13102/sociobiology.v63i1.1025
WIRTH, R. et al. Increasing densities of leaf-cutting ants (Atta spp.) with proximity to the edge in a Brazilian Atlantic forest. Journal of Tropical Ecology, Cambridge, v. 23, p. 501-505, 2007. https://doi.org/10.1017/S0266467407004221
WIRTH, R.; MEYER, S. T.; LEAL, I. R.; TABARELLI, M. Plant-herbivore interactions at the forest edge. Progress in Botany, Berlin, v. 68, p. 423-448, 2008. https://doi.org/10.1007/978-3-540-72954-9_17
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ciência e Natura
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
In particular,
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.
