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Assessment of seed quality and sex determination in hop seedlings using molecular markers

Authors

DOI:

https://doi.org/10.5902/2179460X43135

Keywords:

Breeding programs, Dioecious plants, Humulus lupulus

Abstract

Brazil is the world's third largest beer consumer and currently imports all of its hops for the brewing industry. Such a fact justifies the selection of hop genotypes adapted for cultivation locally, which requires high quality seeds and efficient sex determination of the seedlings. The objectives of this study were to develop a methodology to assess hop seed quality and to efficiently determine hop seedling sex through the use of male-specific molecular markers. Freshly harvested hop seeds were germinated with and without pre-chilling (3-5 ° C) for 3, 6 and 12 weeks and then germinated at 20 or 25 ° C in the presence or absence of light, evaluating germination percentage and germination speed index. F1 progenies were obtained from after seed germination in a greenhouse and seedlings sex was determined using male-specific molecular markers. The best conditions for physiological quality assessment of hop seeds used in the present study were pre-chilling for 12 weeks, followed by germination at 25 ° C, and normal seedling counts at 7 and 15 days. The progeny submitted to molecular marker sexing was composed of 61.3% female plants. The established methodologies presented here can be considered efficient and may contribute to expedite hops breeding programs.

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Author Biographies

Marília Pereira Machado, Universidade Federal do Paraná, Curitiba, PR

Marília Pereira Machado holds your PhD in Science and is currently working as a Professor at Pontifical Catholic University of Parana. Her field of research is focused on biotechnology, plant propagation, secondary metabolism and horticulture.

Andreza Cerioni Belniaki, Universidade Federal do Paraná, Curitiba, PR

Andreza Cerioni Belniaki is an Agronomist and holds your Master's degree in Science. Actually is doing your PhD in Plant Production at Parana Federal University and her field of research is Seed Science and Seed Technology.

André Felipe Bernert, Universidade Federal do Paraná, Curitiba, PR

André Felipe Bernert is an Agronomist Engineer and holds a Master's degree in Science. His research interests include the use of Molecular markers and Plant breeding.

Erik Nunes Gomes, Universidade Federal do Paraná, Curitiba, PR

Erik N. Gomes holds a degree in Agricultural Sciences and a Master's degree in Science and is currently working towards a PhD in Plant Biology, focusing on agronomic approaches to increase the production of plant secondary metabolites.

João Carlos Bespalhok Filho, Universidade Federal do Paraná, Curitiba, PR

Associate Professor at Parana Federal University and his field of research is Breeding and Biotechnology of Crops.

Maristela Panobianco, Universidade Federal do Paraná, Curitiba, PR

Maristela Panobianco is a professor and researcher at Parana Federal University and her field of research is Seed Technology. 

Cícero Deschamps, Universidade Federal do Paraná, Curitiba, PR

Cícero Deschamps is a professor and researcher at Parana Federal University and his field of research is Ecophysiology, Medicinal and Aromatic Plants.

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Published

2020-06-17

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How to Cite

Machado, M. P., Belniaki, A. C., Bernert, A. F., Gomes, E. N., Filho, J. C. B., Panobianco, M., & Deschamps, C. (2020). Assessment of seed quality and sex determination in hop seedlings using molecular markers. Ciência E Natura, 42, e45. https://doi.org/10.5902/2179460X43135

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