Evaluation of bacterial activity in a structured bed reactor under different organic loading rates (OLR)
DOI:
https://doi.org/10.5902/2179460X91578Keywords:
Nitrification, Denitrification, Intermittent aerationAbstract
This research aims to evaluate the bacterial activity response in a continuous upflow structured bed reactor for organic matter and nitrogen removal from dairy wastewater (with an initial concentration of 5,326 mg.L-1 of COD and 39 mg.L-1 of NH4+-N) under different organic loading rates (OLR). An acrylic bench-scale reactor with a total volume of 2.27 L and a working volume of 1.32 L was used and operated for 271 days. The reactor was operated in three experimental phases with different OLRs: Phase I - 1.2 kgCOD.m-3.d-1 (107 days); Phase II - 1.4 kgCOD.m-3.d-1 (94 days); and Phase III - 1.8 kgCOD.m-3.d-1 (70 days). During operation, COD removals above 90% were achieved in all phases. For nitrogen removal, the best results were obtained in Phase I, with average removals of TKN and total nitrogen (TN) of 81% and 74%, respectively. The increase in OLR affected nitrogen removal by directly interfering with the ammonification process. The ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) concentrations decreased with higher OLR. However, despite these results, no accumulation of oxidized nitrogen forms was observed, indicating that nitrification and denitrification occurred simultaneously. The structured bed reactor, operated with recirculation and intermittent aeration, proved effective for simultaneous organic matter and nitrogen removal, creating a stable and favorable environment for combined processes under different OLRs.
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References
American Public Health Association (APHA), American Water Works Association (AWWA), & Water Environment Federation (WEF). (2012). Standard methods for the examination of water and wastewater (22nd ed.). Washington, DC: APHA
Barana, A. C., Lopes, D. D., Martins, T. H., Pozzi, E., Damianovic, M. H. R. Z., Del Nery, V., & Foresti, E. (2013). Nitrogen and organic matter removal in an intermittently aerated fixed-bed reactor for post-treatment of anaerobic effluent from a slaughterhouse wastewater treatment plant. Journal of Environmental Chemical Engineering, 1(3), 453-459. doi: 10.1016/j.jece.2013.06.015.
Begnini, B. C., & Ribeiro, H. B. (2014). Plano para redução de carga poluidora em indústria de lacticínios. Saúde e Meio Ambiente: Revista Interdisciplinar, 3(1), 19-30.
Centro de Estudos Avançados em Economia Aplicada - ESALQ/USP - CEPEA. (2018). Boletim do Leite. Piracicaba. Ano 24 nº 272.
Chiu, Y. C., Lee, L. L., Chang, C. N., & Chao, A. C. (2007). Control of carbon and ammonium ratio for simultaneous nitrification and denitrification in a sequencing batch bioreactor. International Biodeterioration & Biodegradation, 59(1), 1-7. doi: 10.1016/j.ibiod.2006.08.001
Correa, C. Z., Prates, K. V. M. C., De Oliveira, E. F., Lopes, D. D., & Barana, A. C. (2018). Nitrification/denitrification of real municipal wastewater in an intermittently aerated structured bed reactor. Journal of Water Process Engineering, 23, 134-141. doi: 10.1016/j.jwpe.2018.03.013
Correa, .Z., de Tavares Machado Bolonhesi, I.B., Lopes, D.D. et al. (2023). Removal of organic matter and nitrogen from dairy effluents in a structured bed reactor operated with intermittent aeration. Environ Sci Pollut Res 30, 91060–91073 (2023). https://doi.org/10.1007/s11356-023-28581-y
Fu, B., Liao, X., Ding, L., & Ren, H. (2010). Characterization of microbial community in an aerobic moving bed biofilm reactor applied for simultaneous nitrification and denitrification. World Journal of Microbiology and Biotechnology, 26(11), 1981–1990. doi: 10.1007/s11274-010-0382-y.
Gough, R. H., Samkutty, P. J., Mcgrew, P., Arauz, J., & Adkinson, R. W. (2000). Prediction of effluent biochemical oxygen demand in a dairy plant SBR wastewater system. Journal of Environmental Science & Health Part A, 35(2), 169-175. doi: 10.1080/10934520009376961
Hoffmann, H. (2004). Caracterização do funcionamento de ETE do tipo lodo ativado via imagem microscópica–estudo na Região da Grande Florianópolis. XI Simpósio Luso-Brasileiro de Engenharia Sanitária e Ambiental–SILUBESA. Anais, ABES, Natal.
Hui, C., Wei, R., Jiang, H., Zhao, Y., & Xu, L. (2019). Characterization of the ammonification, the relevant protease production and activity in a high-efficiency ammonifier Bacillus amyloliquefaciens DT. International Biodeterioration & Biodegradation, 142, 11-17. doi: 10.1016/j.ibiod.2019.04.009
IBGE, Instituto Brasileiro de Geografia e Estatística. (2022). Available at: https://biblioteca.ibge.gov.br/visualizacao/periodicos/84/ppm_2017_v45_br_informativo.pdf. Accessed on April 29, 2023.
Kushwaha, J. P., Srivastava, V. C., & Mall, I. D. (2011). An overview of various technologies for the treatment of dairy wastewaters. Critical Reviews in Food Science and Nutrition, 51(5), 442-452. doi: 10.1080/10408391003663879
Liu, Y., Shi, H., Xia, L., Shi, H., Shen, T., Wang, Z., Wang, G., & Wang, Y. (2010). Study of operational conditions of simultaneous nitrification and denitrification in a Carrousel oxidation ditch for domestic wastewater treatment. Bioresource Technology, 101(3), 901–906. doi: 10.1016/j.biortech.2009.09.015
Martínez-Suller, L., Provolo, G., Carton, O. T., Brennan, D., Kirwan, L., & Richards, K. G. (2010). The composition of dirty water on dairy farms in Ireland. Irish Journal of Agricultural and Food Research, 67-80.
Mendonça, L. C. (2002). Microbiologia e cinética de sistema de lodos ativados como pós-tratamento de efluente de reator anaeróbio de leito
expandido (Tese de doutorado, Escola de Engenharia de São Carlos, Universidade de São Paulo, São Carlos).
Mille-Lindblom, C., Fischer, H., & Tranvik, L. J. (2006). Antagonism between bacteria and fungi: substrate competition and a possible tradeoff between fungal growth and tolerance towards bacteria. Oikos, 113(2), 233-242. doi: 10.1111/j.2006.0030-1299.14337.x.
Moura, R. B., Damianovic, M. H., & Foresti, E. (2012). Nitrogen and carbon removal from synthetic wastewater in a vertical structured-bed reactor under intermittent aeration. Journal of Environmental Management, 98, 163-167. doi: 10.1016/j.jenvman.2012.01.009
Ødegaard, H., Rusten, B., & Wessman, F. (2004). State of the art in Europe of the moving bed biofilm reactor (MBBR) process. In Proceedings of the 77th Annual Water Environment Federation Technical Exposition and Conference, New Orleans, Louisiana (pp. 2-6).
Schmidt, E. L., & Belser, L. W. (1984). Nitrifying bacteria. In Methods of Soil Analysis – Chemical and Microbiological Properties. Wisconsin, Estados Unidos.
Silva, N. T. M. (2018). Remoção de carbono e nitrogênio de efluente de matadouro em reator anaeróbio-aeróbio de leito fixo (Dissertação de mestrado, Universidade Federal de Pernambuco, Caruaru).
Silva Filho, G. N., & Oliveira, V. L. (2004). Microbiologia: manual de aulas práticas. Ed. da UFSC.
Tiedje, J. (1984). Denitrification. In Methods of Soil Analysis – Chemical and Microbiological Properties. Wisconsin, Estados Unidos.
Wosiack, P. A., Lopes, D. D., Damianovic, M. H. R. Z., Foresti, E., Granato, D., & Barana, A. C. (2015). Removal of COD and nitrogen from animal food plant wastewater in an intermittently-aerated structured-bed reactor. Journal of Environmental Management, 154, 145-150. doi: 10.1016/j.jenvman.2015.02.026
Yonar, T., Sivrioğlu, Ö., & Özengin, N. (2018). Physico-Chemical Treatment of Dairy Industry Wastewaters: A Review. In Technological Approaches for Novel Applications in Dairy Processing (pp. 179).
Zhang, J., & Elser, J. J. (2017). Carbon: nitrogen: phosphorus stoichiometry in fungi: a meta-analysis. Frontiers in Microbiology, 8, 1281. doi: 10.3389/fmicb.2017.01281
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