Microbial enhanced oil recovery (MEOR) by Pseudomonas sp. under laboratory conditions





MEOR, Biotransfomation, Biosurfactant, qPCR


The purpose of this work was to propose sustainable solutions for advanced oil recovery by evaluating the ability of the bacterium Pseudomonas sp. in the biotransformation of alkanes, in addition to determining strain growth patterns under extreme conditions. For this, the work was initially carried out under laboratory conditions, in which the crude oil was fractionated to obtain the saturated fraction used in the experiment. The bacterial tolerance to salinity and temperature was also tested to determine the experimental conditions and set up the experiment in regard to these parameters. Additionally, an experiment was performed to produce a biosurfactant through biostimulation. The biotransformation experiment consisted of a triplicate with treatment and a control. For treatments, Erlenmeyers flasks received 100 mL of broth containing the biosurfactant, 10 g (10%) of NaCl, 3% of the strain and 1% of the saturated fraction. Erlenmeyer flasks were incubated at 40 °C and 180 rpm for 18 days with periodic analysis. The results initially showed the bacteria exhibited better tolerance at a temperature of 40 °C, and there was no significant change for the different salinities, which was a nonlimiting parameter. For the final experiment, the bacterial growth analysed by Optical Density (OD). exhibited a low variation, in which the lowest point was in T18 with an absorbance of 0.115 and the highest point was in T6 with an absorbance of 0.149. In the qPCR analysis of the bacterial population, the pattern found was similar to the optical density results, with low variation; the lowest number of copies of the 16S rRNA gene (6.66x 103) was found in T0 and the highest number was found in T12 (7.86x 103). For biotransformation analysis, time 6 was observed to have the highest rate, with 54% oil recovery (C30), followed by 52% (C31) and 51% (C29).


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Author Biographies

Erick de Aquino Santos, Universidade Federal da Bahia, Salvador, BA

MsC. and PhD. student in Geochemistry of Petroleum and Environmental at Federal University of Bahia 

Manoel Jerônimo Moreira Cruz, Universidade Federal da Bahia, Salvador, BA

PhD. Titular professor of POSPETRO/UFBA. Federal University of Bahia, Geosciences Institute, Oceanography Departament 

Eddy José Francisco de Oliveira, Universidade Estadual de Feira de Santana, Feira de Santana, BA

PhD. Adjunct Professor of the Molecular Biodiversity at UEFS. Universidade Estadual de Feira de Santana (UEFS)

Olivia Maria Cordeiro de Oliveira, Universidade Federal da Bahia, Salvador, BA

PhD. Adjunct Professor of POSPETRO/UFBA. Fedeal University of Bahia (UFBA), Geosciences Institute, Oceanography Departement 

Antônio Fernando de Souza Queiroz, Universidade Federal da Bahia, Salvador, BA

PhD. Adjunct Professor of POSPETRO/UFBA. Federal University of Bahia (UFBA), Geosciences Institute, Oceanography Departement

Sarah Adriana Rocha Soares, Universidade Federal da Bahia, Salvador, BA

PhD. Chemistry in Geosciences Institute, Federal University of Bahia

Danusia Ferreira Lima, Universidade Federal da Bahia, Salvador, BA

PhD. Collaborator teacher of the POSPETRO/UFBA. Federal University of Bahia (UFBA), Geosciences Institute.


AITKEN, C. M.; JONES, D. M.; LARTER, S. R. Anaerobic hydrocarbon biodegradation in deep subsurface oil reservoirs. Nature, v. 431, n. 7006, p. 291-294, 2004.

ASTUTI, Dea Indriani et al. Screening and characterization of biosurfactant produced by Pseudoxanthomonas sp. G3 and its applicability for enhanced oil recovery. Journal of Petroleum Exploration and Production Technology, v. 9, n. 3, p. 2279-2289, 2019.

BROWN, Damon C.; TURNER, Raymond J. Assessing Microbial Monitoring Methods for Challenging Environmental Strains and Cultures. Microbiology Research, v. 13, n. 2, p. 235-257, 2022.

CAPORASO, J. G.; LAUBER, C. L.; WALTERS, W. A.; BERG-LYONS, D.; LOZUPONE, C. A.; TURNBAUGH, P. J.; KNIGHT, R. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proceedings of the national academy of sciences, v. 108, n. supplement_1, p. 4516-4522, 2011.

CHAFALE, Ayushi; KAPLEY, Atya. Biosurfactants as microbial bioactive compounds in microbial enhanced oil recovery. Journal of Biotechnology, 2022.

COTTO, Ada et al. Quantitative polymerase chain reaction for microbial growth kinetics of mixed culture system. Journal of Microbiology and Biotechnology, v. 25, n. 11, p. 1928-1935, 2015.

GAO, H.; ZHANG, J.; LAI, H.; XUE, Q. Degradation of asphaltenes by two Pseudomonas aeruginosa strains and their effects on physicochemical properties of crude oil. International Biodeterioration & Biodegradation, v. 122, p. 12-22, 2017.

GOODMAN, S. H. Investigation into the Potential Application of Microbial Enhanced Oil Recovery on Unconventional Oil: A Field Specific Approach. 2017. 294 f. Tese de Doutorado. University of Liverpool, Liverpool, 2017.

HALIM, A. Y. Application of microorganisms for enhanced oil recovery. 2015. 233 f. Tese de Doutorado (Center for Energy Resources Engineering), Technical University of Denmark, Dinamarca. 2015.

HENTATI, Dorra et al. Investigation of halotolerant marine Staphylococcus sp. CO100, as a promising hydrocarbon-degrading and biosurfactant-producing bacterium, under saline conditions. Journal of Environmental Management, v. 277, p. 111480, 2021.

KE, Cong-Yu et al. A pilot study on large-scale microbial enhanced oil recovery (MEOR) in Baolige Oilfield. International Biodeterioration & Biodegradation, v. 127, p. 247-253, 2018.

KHADEMOLHOSSEINI, Rasoul et al. Investigation of synergistic effects between silica nanoparticles, biosurfactant and salinity in simultaneous flooding for enhanced oil recovery. RSC advances, v. 9, n. 35, p. 20281-20294, 2019.

MOHEBALI, Ghasemali; KAYTASH, Ashk; ETEMADI, Narges. Efficient breaking of water/oil emulsions by a newly isolated de-emulsifying bacterium, Ochrobactrum anthropi strain RIPI5-1. Colloids and Surfaces B: Biointerfaces, v. 98, p. 120-128, 2012.

NAZINA, T. N.; GRIGOR’YAN, A. A.; SHESTAKOVA, N. M.; BABICH, T. L.; IVOILOV, V. S.; FENG, Q.; NI, F.; WANG, J.; SHE, Y.; XIANG, T.LUO, Z.; BELYAEV, S. S.; IVANOV, M. V. Microbiological investigations of high-temperature horizons of the Kongdian petroleum reservoir in connection with field trial of a biotechnology for enhancement of oil recovery. Microbiology, v. 76, n. 3, p. 287-296, 2007.

NIU, J.; LIU, Q.; LV, J.; PENG, B. Review on microbial enhanced oil recovery: Mechanisms, modeling and field trials. Journal of Petroleum Science and Engineering. p. 107350, 2020.

OKORO, Emmanuel E.; EFAJEMUE, E. A.; SANNI, S. E.; OLABODE, O. A.; ORODU, O. D.; OJO, T. Application of thermotolerant petroleum microbes at reservoir conditions for enhanced oil recovery. Petroleum, 2022.

PARADA, ALMA E.; NEEDHAM, DAVID M.; FUHRMAN, JED A. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environmental microbiology, v. 18, n. 5, p. 1403-1414, 2016.

RICOMARTÍNEZ, R., SNELL, T.W., SHEARER, T.L. Synergistic toxicity of Macondo crude oil and dispersant Corexit 9500A® to the Brachionus plicatilis species complex (Rotifera). Environ. Pollut. v. 173, n.173, p. 5-10, 2013.

SHE, H.; KONG, D.; LI, Y.; HU, Z.; GUO, H. Recent advance of microbial enhanced oil recovery (MEOR) in China. Geofluids, v. 2019, 2019.

SHIBULAL, B.; AL-BAHRY, S. N.; AL-WAHAIBI, Y. M.; ELSHAFIE, A. E.; AL-BEMANI, A. S.; JOSHI, S. J. Microbial enhanced heavy oil recovery by the aid of inhabitant spore-forming bacteria: an insight review. The Scientific World Journal, v. 2014, 2014.

SHIBULAL, Biji et al. Microbial-enhanced heavy oil recovery under laboratory conditions by Bacillus firmus BG4 and Bacillus halodurans BG5 isolated from heavy oil fields. Colloids and Interfaces, v. 2, n. 1, p. 1, 2018.

TIAN, L., BAI, Y.L., ZHONG, J.J. Recent developments in the degradation of toxic organic pollution by microorganisms. J. Ind. Microbl. V. 3, n. 2, p. 46-50, 2000.

YERNAZAROVA, A.; KAYIRMANOVA. G.; BAUBEKOVA, A.; ZHUBANOVA, A. Microbial Enhanced Oil Recovery. In: ROMERO-ZERON, L. (Ed.). Chemical Enhanced Oil Recovery (cEOR) - a Practical Overview, InTech, 2016. p. 2006.

YOUSSEF, N.; ELSHAHED, M. S.; MCINERNEY, M. J. In: Advances in Applied Microbiology. Microbial processes in oil fields: culprits, problems, and opportunities, v. 66, p. 141-251, 2009.



How to Cite

Santos, E. de A., Cruz, M. J. M., Oliveira, E. J. F. de, Oliveira, O. M. C. de, Queiroz, A. F. de S., Soares, S. A. R., & Lima, D. F. (2023). Microbial enhanced oil recovery (MEOR) by Pseudomonas sp. under laboratory conditions. Revista Eletrônica Em Gestão, Educação E Tecnologia Ambiental, 26, e11. https://doi.org/10.5902/2236117071814




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