Isolation of extended-spectrum beta-lactamases producing strains on effluent of state hospital from Boa Vista-RR
DOI:
https://doi.org/10.5902/2236117029815Keywords:
Antimicrobial, Resistance, ESBL, Environment.Abstract
The extended spectrum beta-lactamase (ESBL) producing bacteria are widespread in hospital environments. The incorrect disposal of clinical waste in hospital effluents, allows them to present high concentrations of bacteria with antimicrobial resistance profiles that present an environmental risk to the extent that they allow the dissemination. This research covers the characterization of the presence of multi-resistant antimicrobial bacteria in strains isolated from hospital effluents, whose associated risks include the spread of pathogenic microorganisms in the environment. To complete this research, the proposed objectives were: characterization of the hospital effluent strains generated by the General Hospital of Roraima (GHR) and research of the resistance genes responsible for ESBL coding and hydrolysis to the antimicrobials of the class of cephalosporins, penicillins and monobactams. Samples were collected from the hospital septic tank from June 2014 to June 2016, submitted to molecular identification for the detection of genes through the polymerase chain reaction (PCR). The results showed the presence of ESBL in all of the collected tanks and a multi-resistance profile. ESBL genes were identified on isolated frequencies and they were combined. The results indicated the widespread dissemination of the ESBL genes in the GHR effluent.Downloads
References
ABREU, E.T. et al., Avaliação da resistência a antibióticos de bactérias isoladas de efluente hospitalar. Acta Scientiarum. Technology, v. 32, n. 1, p. 1-5, 2010.
ANVISA – AGÊNCIA NACIONAL DE VIGILÂNCIA SANITÁRIA. Resolução RDC nº. 306/2004. Dispõe sobre o regulamento técnico para o gerenciamento de resíduos de serviço de saúde. Diário Oficial da União 10/12/2004.
ASSOCIAÇÃO BRASILEIRA DE NORMAS TÉCNICAS. NBR 9898: Preservação e técnicas de amostragem de efluentes líquidos e corpos receptores. Rio de Janeiro, 1987.
BRASIL. Agência Nacional de Vigilância Sanitária (ANVISA). Gerência de Investigação e Prevenção das Infecções e dos Eventos Adversos (Gipea) e Gerência Geral de Tecnologia em Serviços de Saúde (GGTES). Investigação e Controle de bactérias Multirresistentes. Brasília: ANVISA, 2007. 21 p. Disponível em: <http://www.anvisa.gov.br/servicosaude/controle/reniss/manual%20_controle_ bacterias.pdf>. Acesso em: 15 maio 2015.
CARMO.S.D et al, Detecção de Betalactamases de espectro estendido (ESBL) em isolados de escherichia coli uropatogênicas (UPECS) oriundos de pacientes da comunidade. Revista de Patologia Tropical, v.41, n.4, p.419-426, out.-dez. 2012.
CAVALCANTI, Dany Geraldo Kramer et al. Caracterização físico-química e microbiológica de efluente hospitalar na Região Metropolitana do Natal-RN-Brasil. Revista de Biologia e Ciências da Terra, v. 11, n. 1, p. 205-210, 2011.
CHAGAS T.P.G. et al, Multiresistance, beta-lactamase-encoding genes and bacterial diversity in hospital wastewater in Rio de Janeiro, Brazil J. Appl. Microbiol., v. 111 p. 572–581, 2011.
CHAPMAN, P. A. et al. Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products. International Journal of Food Microbiology, v. 68, n. 1, p. 11-20, ago. 2001.
CLSI. Performance Standards for Antimicrobial Sucetibility Testing- Twenty Second Information Supplement. M100-S22. Clinical Laboratory and Standard Institute, v.32, n.3, Jan 2012.
DRIEUX, Laurence et al. Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain. Antimicrobial Resistance & Infection Control, v. 5, n. 1, p. 1, 2016.
FERREIRA, Cristina Motta et al. Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil. Brazilian Journal of Microbiology, v. 42, n. 3, p. 1076-1084, 2011.
GUSATTI C., et al, Resistência a β-lactâmicos em Acinetobacter spp isolados de efluente hospitalar no sul do Brasil. Rev. Soc. Bras. Med. Trop. v.42, n.2 Mar./Apr. 2009.
KORZENIWESKA E., HARNISZ. Beta-lactamase-producing Enterobacteriaceae in hospital effluents.. Journal of enviromental management, v. 123, p 1–7, july 2013.
LEISTNER, R. et al, Risk Factors Associated with the Community-Acquired Colonization of Extended-Spectrum Beta-Lactamase (ESBL) Positive Escherichia Coli.An Exploratory Case Control Study. PLOS ONE, v, 8, n. 9, sept 2013.
LIVERMORE, D.M. et, al. Are susceptibility tests enough, or should laboratories still seek ESBLs and carbapenemases directly? Antimicrob. Agents Chemother. Advanced Acess. March 2012. .
MUNIER, G.K, Positive Extended-Spectrum--Lactamase (ESBL) Screening Results May Be Due to AmpC-Lactamases More Often than to ESBL. Journal of Clinical Microbiology, v.48, n.2, p. 673-674, feb 2010.
OVERDEVEST, I. Extended-Spectrum β-Lactamase Genes of Escherichia coli in Chicken Meat and Humans, the Netherlands. Emerging Infectious Diseases, v. 17, n.7, p.1216-1222, July 2011.
PATTARACHAI et al. Molecular Characterization and Epidemiology of Extended-Spectrum--Lactamase-Producing Escherichia coli and Klebsiella pneumonia Isolates Causing Health Care-Associated Infection in Thailand,Where the CTX-M Family Is Endemic. Antimicrobial Agents and Chemoterapy, v. 5, n.8, p-2818-2824, 2008.
PRADO, T., et al., Detection of extended-spectrum b-lactamase-producing Klebsiella pneumoniae in effluents and sludge of a hospital sewage treatment plant. Letters in Applied Microbiology, v 46, p 136-141, 2008.
REINTHALER, F.F, et al. ESBL-producing E. coli in Austrian sewage sludge. Water Research, v. 44, p. 1981-1985, March 2010.
RORAIMA. Plano de Gerenciamento de Resíduos de Serviços de Saúde – PGRSS. Secretaria de Estado da Saúde. Hospital Geral de Roraima, 48p, Roraima, 2013.
SILVA, D.G.K.C; et al. Caracterização físico-química e microbiológica de efluente hospitalar na Região Metropolitana de Natal-RN – Brasil. Revista de Biologia e Ciências da Terra, v. 11, n. 1,p 205-210, 2011.
SILVA, K.C.; LINCOPAN, N., Epidemiologia das betalactamases de espectro estendido no Brasil: impacto clínico e implicações para o agronegócio. J Bras Patol Med Lab, v. 48, n. 2, p. 91-99 , abril 2012.
TACÃO, M. et al. Co-resistance to different classes of antibiotics among ESBL-producers from aquatic systems, Water Research, v. 48, p-100-107, 2014.
VECCHIA, A.D. et al. Diagnóstico sobre a situação do tratamento do esgoto hospitalar no Brasil. Revista Saúde e Ambiente / Health and Environment Journal, v. 10, n. 2, dez. 2009.
WANI, K. A. et al. Extended Spectrum-Lactamase Mediated Resistance in Escherichia Coli in a Tertiary Care. International journal of health sciences, v. 3, n. 2, 2010.
ZARFEL G. et al, Comparison of extended-spectrum-β-lactamase (ESBL) carrying Escherichia coli from sewage sludge and human urinary tract infection. Environmental Pollution, v. 137, p; 192-199, Feb 2013.
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