Case Report: Cross-reaction of phencyclidine in immunochromatographic tests for rapid drug detection using in silico analysis for investigation

Authors

DOI:

https://doi.org/10.5902/2236583475385

Keywords:

Immunochromatography, Case report, Phencyclidine, In silico analysis, Cross-reaction

Abstract

Phencyclidine (PCP) is a dissociative drug previously used as an anesthetic agent but caused hallucinations due to its neurotoxic effects. A 49-year-old male farmer attempted suicide at home due to a massive intake of prescription drugs. Toxicological analysis was performed with an immunochromatography test and detected PCP (an uncommon drug in Brazil). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and in silico models were employed to elucidate this case. Despite the presence of midazolam, fentanyl and lidocaine in urine and serum samples by LC-MS/MS, these drugs and respective metabolites were not able to promote a cross-reaction with PCP as demonstrated by in silico models as well as our practical experience. In fact, doxepin, trimipramine and promethazine and their respective metabolites showed a high correlation interaction in the binding site of 2PCP, especially promethazine. Predicted results obtained on docking show a high correlation interaction between PCP and promethazine, indicating a possible cross-reaction in the immunochromatography test.

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Author Biographies

Marcelo Henrique Santana Nascimento, Federal University of Santa Maria

Student of the Pharmacy at the Federal University of Santa Maria (UFSM). Has experience in Pharmacology, with emphasis on Toxicology, acting on the following subjects: HPLC UV/VIS, tricyclic antidepressants.

Leonardo Correa Cardoso, Federal University of Santa Maria

Graduated in Pharmacy from the Federal University of Santa Maria (2017). Working mainly on the following topics: Pharmacognosy, Hospital Pharmacy, Chemotherapy Manipulation, Quality Control of Biological Medicines (ICPO, APTT, LAL, Factor IIa and Xa, Acrylamide gel electrophoresis and Isoelectric focusing), Pharmaceutical Technology, Cosmetology and Nanotechnology.

Rachel Santos, Federal University of Santa Maria

Student of the Pharmacy course at the Federal University of Santa Maria (UFSM). Currently, she performs Scientific Initiation at the Nucleus Applied to Toxicology (NAT). Member of the Brazilian Society of Toxicology (SBTox).

Miguel Roehrs, Hospital Universitário de Santa Maria

Graduated in Pharmacy and Biochemistry with an emphasis in Clinical and Toxicological Analysis from the Federal University of Santa Maria (2006). He obtained a master's degree in Pharmacology from the Federal University of Santa Maria (2009) and a Ph.D. in Pharmacology in 2014.

Sarah Carobini Werner de Souza Eller Franco de Oliveira, Health Sciences Federal University of Porto Alegre

Master's Degree in Toxicology and Toxicological Analysis from the University of São Paulo (2014). Ph.D. in the Graduate Program in Toxicology and Toxicological Analysis at USP (2018).

Tiago Franco de Oliveira, Health Sciences Federal University of Porto Alegre

Ph.D. in Toxicology and Toxicological Analysis (2012) and a postdoctoral degree in Toxicology (2013-2017). He is currently an Adjunct Professor at the Federal University of Health Sciences of Porto Alegre (UFCSPA), in the Knowledge Area of Analytical, Environmental, Forensic and Social Toxicology.

André Valle de Bairros, Federal University of Santa Maria

Degree in Pharmacy (2007), Master's Degree in Toxicological Biochemistry (2009) from the Federal University of Santa Maria (UFSM) and Ph.D. in Toxicology and Toxicological Analysis from the University of São Paulo (USP).

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Published

2024-02-27

How to Cite

Nascimento, M. H. S., Cardoso, L. C., Santos, R., Roehrs, M., Oliveira, S. C. W. de S. E. F. de, Oliveira, T. F. de, & Bairros, A. V. de. (2024). Case Report: Cross-reaction of phencyclidine in immunochromatographic tests for rapid drug detection using in silico analysis for investigation. Saúde (Santa Maria), 49(2), e75385. https://doi.org/10.5902/2236583475385

Issue

Vol. 49 No. 2 (2023): Revista Saúde (Santa Maria) Ano 2023

Section

Artigos

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Copyright (c) 2024 Marcelo Henrique Santana Nascimento, Leonardo Correa Cardoso, Rachel Santos, Miguel Roehrs, Sarah Carobini Werner de Souza Eller Franco de Oliveira, Tiago Franco de Oliveira, André Valle de Bairros

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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